Detection of High Molecular Weight Glutenin Subunits in Triticale (x Triticosecale Wittm.) Cultivars by Capillary Zone Electrophoresis

被引:10
作者
Salmanowicz, Boleslaw P. [1 ]
机构
[1] Polish Acad Sci, Inst Plant Genet, PL-60479 Poznan, Poland
关键词
Allelic variation; Glu-1; loci; storage proteins; triticale; wheat;
D O I
10.1021/jf8016546
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
An improved method for separating and characterizing high molecular weight glutenin subunits (HMW-GS) in hexaploid triticale by capillary zone electrophoresis (CZE) was developed. A low-concentrate mixture of hydrophilic polymers, poly(vinylpyrrolidone) (PVP) and hydroxypropylmethylcellulose (HPMC), in an isoelectric buffer was employed for dynamic coating of the capillary inner wall. In separation buffer PVP with lower concentrated poly(ethylene oxide) (PEO) was replaced. The CZE electropherograms of HMW-GS showed two group peaks in accordance with x- and y-type subunits with migration times of 6.8-7.8 and 8.4-11.5 min, respectively. In total, 14 HMW subunits (2 subunits encoded by Glu-A1 locus and 12 by Glu-B1) were identified. The CZE analyses revealed that each of the subunits Bx7 and By8 determined by SIDS-PAGE makes up three subunits (Bx6.8, Bx7, and Bx7* and By8, By8*, and new By8**, respectively), with different migration times. It was also shown that the subunits By18 and By20 in triticale determined by SDS-PAGE have different migration times in comparison with the same subunits in bread wheat. For these new HMW-GS, the following names were assigned: By18* instead of By18 and By20* instead of By20. The presented CZE method is an efficient alternative to the SIDS-PAGE procedure for early selection of useful triticale genotypes with good breadmaking quality.
引用
收藏
页码:9355 / 9361
页数:7
相关论文
共 37 条
[1]   Diversity of seven glutenin and secalin loci within triticale cultivars grown in Europe [J].
Amiour, N ;
Bouguennec, A ;
Marcoz, C ;
Sourdille, P ;
Bourgoin, M ;
Khelifi, D ;
Branlard, G .
EUPHYTICA, 2002, 123 (03) :295-305
[2]   Allelic variation of HMW and LMW glutenin subunits, HMW secalin subunits and 75K gamma-secalins of hexaploid triticale [J].
Amiour, N ;
Dardevet, A ;
Khelifi, D ;
Bouguennec, A ;
Branlard, G .
EUPHYTICA, 2002, 123 (02) :179-186
[3]   Environmental stability and heritability estimates for grain yield and test weight in triticale [J].
Barnett, Ronald D. ;
Blount, Ann R. ;
Pfahler, Paul L. ;
Bruckner, Philip L. ;
Wesenberg, Darrell M. ;
Johnson, Jerry W. .
JOURNAL OF APPLIED GENETICS, 2006, 47 (03) :207-213
[4]   Sodium dodecyl sulfate capillary electrophoresis of wheat proteins. 1. Uncoated capillaries [J].
Bean, SR ;
Lookhart, GL .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (10) :4246-4255
[5]   Ultrafast capillary electrophoretic analysis of cereal storage proteins and its applications to protein characterization and cultivar differentiation [J].
Bean, SR ;
Lookhart, GL .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (02) :344-353
[6]   Faster capillary electrophoresis separation of wheat proteins through modifications to buffer composition and sample handling [J].
Bean, SR ;
Lookhart, GL .
ELECTROPHORESIS, 1998, 19 (18) :3190-3198
[7]   Optimizing quantitative reproducibility in high-performance capillary electrophoresis (HPCE) separations of cereal proteins [J].
Bean, SR ;
Lookhart, GL .
CEREAL CHEMISTRY, 2001, 78 (05) :530-537
[8]  
BRZEZINSKI W, 2006, SUPIA WIELKA, V81
[9]   Separation of double-stranded DNA fragments by capillary electrophoresis: Impacts of poly(ethylene oxide), gold nanoparticles, ethidium bromide, and pH [J].
Chiou, SH ;
Huang, MF ;
Chang, HT .
ELECTROPHORESIS, 2004, 25 (14) :2186-2192
[10]   Comparison of the separation of large DNA fragments in the presence and absence of electroosmotic flow at high pH [J].
Chiu, TC ;
Chang, HT .
JOURNAL OF CHROMATOGRAPHY A, 2002, 979 (1-2) :299-306