Regrowth of enterococci indicator in an open recycled-water impoundment

The purpose of the research was to assess the potential for enterococci faecal-indicator to regrow in recycled water while under environmentally-open storage. Regrowth would result in false-positive indicator results with possible downgrading, rejection or over-chlorination of recycled water. The research setting was the main 93-megalitre storage impoundment of the Hawkesbury Water Recycling Scheme in Sydney's North West, receiving tertiary treated (chlorinated) effluent from the Richmond sewage treatment plant. The water is used to irrigate horticultural food crops, pasture for dairy cattle, sheep, deer and horses, and for the maintenance of lawns and sports fields. Highly significant positive relationships were noted in multivariate analysis between indicator counts and the growth factors atmospheric temperature and UV254 (unfiltered) as proxy for total organic carbon (p = 0.001 and 0.003 respectively). Nitrate and phosphate did not show significant relationships suggesting that these nutrients may not be growth-limiting at levels found in recycled water. Rainfall and wild duck presence did not appear to have an impact on enterococcal growth in the study. The overall predictive power of the regression model was shown to be highly significant (p = 0.001). These findings will assist in recycled water monitoring and the revision of guidelines, with potential for the reduction of the chlorination byproduct burden on the environment. A formula derived for the relationship between the indicator and atmospheric temperature could be used in food-production and climate-change modelling. (C) 2013 Elsevier B.V. All rights reserved.