Time Course Analysis Reveals Gene-Specific Transcript and Protein Kinetics of Adaptation to Short-Term Aerobic Exercise Training in Human Skeletal Muscle

被引:96
作者
Egan, Brendan [1 ,2 ]
O'Connor, Paul L. [3 ]
Zierath, Juleen R. [2 ,4 ]
O'Gorman, Donal J. [3 ,5 ]
机构
[1] Univ Coll Dublin, Sch Publ Hlth Physiotherapy & Populat Sci, Inst Sport & Hlth, Dublin 2, Ireland
[2] Karolinska Inst, Sect Integrat Physiol, Dept Mol Med & Surg, Stockholm, Sweden
[3] Dublin City Univ, Sch Hlth & Human Performance, Dublin 9, Ireland
[4] Karolinska Inst, Dept Physiol & Pharmacol, Stockholm, Sweden
[5] Dublin City Univ, Ctr Prevent Med, Dublin 9, Ireland
基金
欧洲研究理事会; 瑞典研究理事会;
关键词
INDUCED MITOCHONDRIAL BIOGENESIS; MESSENGER-RNA ABUNDANCE; ALPHA ERR-ALPHA; ENDURANCE EXERCISE; RECEPTOR-ALPHA; BIOCHEMICAL ADAPTATIONS; METABOLIC ADAPTATIONS; CONTRACTILE ACTIVITY; ENERGY-METABOLISM; COACTIVATOR PGC-1;
D O I
10.1371/journal.pone.0074098
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Repeated bouts of episodic myofibrillar contraction associated with exercise training are potent stimuli for physiological adaptation. However, the time course of adaptation and the continuity between alterations in mRNA expression and protein content are not well described in human skeletal muscle. Eight healthy, sedentary males cycled for 60 min at 80% of peak oxygen consumption (VO2peak) each day for fourteen consecutive days, resulting in an increase in VO2peak of 17.5 +/- 3.8%. Skeletal muscle biopsies were taken at baseline, and on the morning following (+16 h after exercise) the first, third, seventh, tenth and fourteenth training sessions. Markers of mitochondrial adaptation (Cyt c and COXIV expression, and citrate synthase activity) were increased within the first week of training, but the mtDNA/nDNA ratio was unchanged by two weeks of training. Accumulation of PGC-1 alpha and ERR alpha protein during training suggests a regulatory role for these factors in adaptations of mitochondrial and metabolic gene expression. A subset of genes were transiently increased after one training session, but returned to baseline levels thereafter, which is supportive of the concept of transcriptional capacity being particularly sensitive to the onset of a new level of contractile activity. Thus, gene-specific temporal patterns of induction of mRNA expression and protein content are described. Our results illustrate the phenomenology of skeletal muscle plasticity and support the notion that transcript level adjustments, coupled to accumulation of encoded protein, underlie the modulation of skeletal muscle metabolism and phenotype by regular exercise.
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页数:13
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