First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA

被引：383

作者：

Sherchan, Samendra P. ^{[1
]}

Shahin, Shalina ^{[1
]}

Ward, Lauren M. ^{[1
]}

Tandukar, Sarmila ^{[2
]}

Aw, Tiong G. ^{[1
]}

Schmitz, Bradley ^{[3
]}

Ahmed, Warish ^{[4
]}

Kitajima, Masaaki ^{[5
]}

机构：

[1] Tulane Univ, Dept Environm Hlth Sci, 1440 Canal St,Suite 2100, New Orleans, LA 70112 USA

[2] Univ Yamanashi, Interdisciplinary Ctr River Basin Environm, 4-3-11 Takeda, Kofu, Yamanashi 4008511, Japan

[3] Loudoun Water, 44865 Loudoun Water Way, Ashburn, VA 20147 USA

[4] CSIRO Land & Water, Ecosci Precinct, 41 Boggo Rd, Dutton Pk, Qld 4102, Australia

[5] Hokkaido Univ, Fac Engn, Div Environm Engn, Kita Ku, North 13 West 8, Sapporo, Hokkaido 0608628, Japan

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 2020年 / 743卷

基金：

日本学术振兴会;

关键词：

Wastewater-based epidemiology; SARS-CoV-2; Surveillance; COVTD-19; RT-qPCR; Wastewater; REAL-TIME PCR; CORONAVIRUS;

D O I：

10.1016/j.scitotenv.2020.140621

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

We investigated the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater samples in southern Louisiana, USA. Untreated and treated wastewater samples were collected on five occasions over a four-month period from January to April 2020. The wastewater samples were concentrated via ultrafiltration (Method A). and an adsorption-elution method using electronegative membranes (Method B). SARS-CoV-2 RNA was detected in 2 out of 15 wastewater samples using two reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays (CDC N1 and N2). None of the secondary treated and final effluent samples tested positive for SARS-CoV-2 RNA. To our knowledge, this is the first study reporting the detection of SAR.S-CoV-2 RNA in wastewater in North America, including the USA. However, concentration methods and RTqPCR assays need to be refined and validated to increase the sensitivity of SARS-CoV-2 RNA detection in wastewater. (C) 2020 Elsevier B.V. All rights reserved.

引用

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