Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions

被引:8
作者
Zanchetta, Flavia Cristina [1 ]
Trinca, Rafael Bergamo [2 ]
Gomes Silva, Juliany Lino [1 ]
Cunha Breder, Jessica da Silva [1 ]
Cantarutti, Thiago Anselmo [1 ]
Consonni, Silvio Roberto [3 ]
Moraes, Angela Maria [2 ]
de Araujo, Eliana Pereira [1 ]
Abdalla Saad, Mario Jose [4 ]
Adams, Gary G. [5 ]
Melo Lima, Maria Helena [1 ]
机构
[1] Univ Estadual Campinas, Sch Nursing, BR-13083887 Campinas, Brazil
[2] Univ Estadual Campinas, Sch Chem Engn, Dept Engn Mat & Bioproc, BR-13083852 Campinas, Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, BR-13083970 Campinas, Brazil
[4] Univ Estadual Campinas, Dept Internal Med, BR-13083887 Campinas, Brazil
[5] Univ Nottingham, Fac Med, Queens Med Ctr, Sch Hlth Sci, C Floor,South Block Link, Nottingham NG7 2HA, England
基金
巴西圣保罗研究基金会;
关键词
C57BL; 6J mice; wound healing; wound dressing; chitosan; polycaprolactone; electrospinning; POLY(ETHYLENE GLYCOL); WOUND REPAIR; CHITOSAN; BIOMATERIALS; ALPHA; INFLAMMATION; NANOFIBERS; SCAFFOLDS; DRESSINGS; NETWORKS;
D O I
10.3390/polym12071580
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Polycaprolactone (PCL) is a synthetic polymer with good mechanical properties that are useful to produce biomaterials of clinical application. It can be successfully combined with chitosan, which enhances the biomaterial properties through the modulation of molecular and cellular mechanisms. The objective of this study was to evaluate the effects of the use of electrospun fibrous membranes consisting of polycaprolactone (PCL) or polycaprolactone coated with chitosan and poly(ethylene oxide) (PCL+CHI/PEO) on mouse skin lesions. Sixty four Black-57 mice were divided into PCL and PCL+CHI/PEO groups. A 1 cm(2)lesion was made on the animals' backs, and the membranes were sutured in place. The tissues were extracted on the 3rd, 7th, and 14th days after the lesion. The tissues were analyzed by histology with Hematoxylin and Eosin (H&E) and Sirius Red stains, morphometry, immunohistochemistry, and Western blot. On the 3rd, 6th, and 9th days after the lesion, the PCL+CHI/PEO group showed a higher wound-healing rate (WHR). On the 3 day, the PCL+CHI/PEO group showed a greater amount of inflammatory infiltrate, greater expression of proliferating cell nuclear antigen (PCNA), and smooth muscle actin (alpha-SMA) (p< 0.05) compared to the PCL group. On the 7th day after the lesion, the PCL+CHI/PEO group showed a greater amount of inflammatory infiltrate, expression of Tumor Necrosis Factor (TNF-alpha) and PCNA (p< 0.05). In addition, it showed a greater immunolabeling of Monocyte Chemoattractant Protein-1 (MCP-1) and deposition of collagen fibers compared to the PCL group. The PCL+CHI/PEO membrane modulated the increase in the inflammatory infiltrate, the expression of MCP-1, PCNA, and alpha-SMA in lesions of mice.
引用
收藏
页码:1 / 18
页数:18
相关论文
共 59 条
[1]   Antimicrobial wound dressing nanofiber mats from multicomponent (chitosan/silver-NPs/polyvinyl alcohol) systems [J].
Abdelgawad, Abdelrahman M. ;
Hudson, Samuel M. ;
Rojas, Orlando J. .
CARBOHYDRATE POLYMERS, 2014, 100 :166-178
[2]   Electrospun poly(ε-caprolactone)-based skin substitutes: In vivo evaluation of wound healing and the mechanism of cell proliferation [J].
Augustine, Robin ;
Dominic, Edwin Anto ;
Reju, Indu ;
Kaimal, Balarama ;
Kalarikkal, Nandakumar ;
Thomas, Sabu .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2015, 103 (07) :1445-1454
[3]   Chitosan: A potential biopolymer for wound management [J].
Bano, Ijaz ;
Arshad, Muhammad ;
Yasin, Tariq ;
Ghauri, Muhammad Afzal ;
Younus, Muhammad .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2017, 102 :380-383
[4]   Growth factors and cytokines in wound healing [J].
Barrientos, Stephan ;
Stojadinovic, Olivera ;
Golinko, Michael S. ;
Brem, Harold ;
Tomic-Canic, Marjana .
WOUND REPAIR AND REGENERATION, 2008, 16 (05) :585-601
[5]  
Bombaldi de Souza., 2020, Biopolymer Membranes and Films, P165, DOI [DOI 10.1016/B978-0-12-818134-8.00007-9, 10.1016/B978-0-12-818134-8.00007-9, 10.1016/B978-0-12818134-8.00007-9, DOI 10.1016/B978-0-12818134-8.00007-9]
[6]   INCORPORATION AND RELEASE KINETICS OF ALPHA-BISABOLOL FROM PCL AND CHITOSAN/GUAR GUM MEMBRANES [J].
Bombaldi de Souza, F. C. ;
Bombaldi de Souza, R. F. ;
Moraes, A. M. .
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING, 2016, 33 (03) :453-467
[7]   Chitosan-alginate membranes accelerate wound healing [J].
Caetano, Guilherme Ferreira ;
Cipriani Frade, Marco Andrey ;
Moretti Andrade, Thiago Antonio ;
Leite, Marcel Nani ;
Bueno, Cecilia Zorzi ;
Moraes, Angela Maria ;
Ribeiro-Paes, Joao Tadeu .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2015, 103 (05) :1013-1022
[8]   Bio-Conjugated Polycaprolactone Membranes: A Novel Wound Dressing [J].
Cai, Elijah Zhengyang ;
Teo, Erin Yiling ;
Jing, Lim ;
Koh, Yun Pei ;
Qian, Tan Si ;
Wen, Feng ;
Lee, James Wai Kit ;
Hing, Eileen Chor Hoong ;
Yap, Yan Lin ;
Lee, Hanjing ;
Lee, Chuen Neng ;
Teoh, Swee-Hin ;
Lim, Jane ;
Lim, Thiam Chye .
ARCHIVES OF PLASTIC SURGERY-APS, 2014, 41 (06) :638-646
[9]   Indirect effects of immunological tolerance to a regular dietary protein reduce cutaneous scar formation [J].
Cantaruti, Thiago Anselmo ;
Costa, Raquel Alves ;
de Souza, Kenia Soares ;
Vaz, Nelson Monteiro ;
Carvalho, Claudia Rocha .
IMMUNOLOGY, 2017, 151 (03) :314-323
[10]   Electrospun chitosan/polyvinyl alcohol nanofibre mats for wound healing [J].
Charernsriwilaiwat, Natthan ;
Rojanarata, Theerasak ;
Ngawhirunpat, Tanasait ;
Opanasopit, Praneet .
INTERNATIONAL WOUND JOURNAL, 2014, 11 (02) :215-222