Screening of Brazilian medicinal plants for antiviral activity against rotavirus

被引：68

作者：

Cecilio, Alzira Batista ^{[1
]}

de Faria, Deborah Behr ^{[1
]}

Oliveira, Pollyana de Carvalho ^{[1
]}

Caldas, Sergio ^{[1
]}

de Oliveira, Dario Alves ^{[2
]}

Sobral, Marcos Eduardo Guerra ^{[3
]}

Duarte, Maria Gorette Resende ^{[1
]}

Moreira, Carolina Paula de Souza ^{[1
]}

Silva, Claudia Gontijo ^{[1
]}

de Almeida, Vera Lucia ^{[1
]}

机构：

[1] Fundacao Ezequiel Dias, Diretoria Pesquisa Desenvolvimento, Rua Conde Pereira Carneiro,80,Gameleira, BR-30510010 Belo Horizonte, MG, Brazil

[2] Univ Estadual Montos Claros, Ctr Crencias Biol & Sande, BR-39401089 Montes Claros, MG, Brazil

[3] Univ Fed Sao Joeio De Rei, Dept Ciencias Nat, BR-36301160 Sao Joao Del Rei, MG, Brazil

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2012年 / 141卷 / 03期

关键词：

Medicinal plants; Cytotoxicity; Antiviral activity; Rotavirus; IN-VITRO; SIMIAN ROTAVIRUS; VIRAL GASTROENTERITIS; HYMENAEA-COURBARIL; CHILD-MORTALITY; EXTRACTS; FLAVONOIDS; DIARRHEA; VIRUS; SA11;

D O I：

10.1016/j.jep.2012.03.031

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ethnopharmacological relevance: Brazilian medicinal plants traditionally used for the treatment of diarrhoea were investigated for their in vitro antiviral activity against the simian rotavirus SA11. Materials and methods: The ethanolic crude extracts of plants collected in the cerrado of Minas Gerais, Brazil were submitted to phytochemical screening. The cytotoxicity of the extracts was inferred by cellular morphologic alterations. Antiviral activity was assessed by the ability of the extracts to inhibit the cytopathic effect (CPE) of rotavirus on the treated cells. RT-PCR was performed to confirm and/or confront antiviral assay data. Results: The maximum non-toxic concentration ranged from 50 to 500 mu g/mL. All extracts were toxic at a concentration of 5000 mu g/mL but no extract showed cytotoxicity at 50 mu g/mL. The species Byrsonima verbascifolia, Myracrodruon urundeuva, Eugenia dysenterica and Hymenaea courbaril exhibited the strongest in vitro activity against rotavirus. Their extracts prevented the formation of CPE, and RT-PCR analysis detected no amplification of genetic material from rotavirus. Tannins, flavonoids, saponins, coumarins and terpenes were the major classes of natural products found in the leaf extracts that showed antiviral activity. Conclusion: Among the species studied, Byrsonima verbascifolia, Eugenia dysenterica, Hymenaea courbaril and Myracrodruon urundeuva showed potential activity against rotavirus and are worthy of further study. The present study corroborates ethnopharmacological data as a valuable source in the selection of plants with antiviral activity and to some extent validates their traditional uses. Published by Elsevier Ireland Ltd.

引用

页码：975 / 981

页数：7

共 59 条

[1] Isolation and absolute configuration of ent-halimane diterpenoids from Hymenaea courbaril from the suriname rain forest [J].