Macrolide resistance genotypes and phenotypes among erythromycin-resistant clinical isolates of Staphylococcus aureus and coagulase-negative staphylococci, Italy

被引：33

作者：

Gherardi, Giovanni ^{[1
]}

De Florio, Lucia ^{[1
]}

Lorino, Giulia ^{[2
]}

Fico, Laura ^{[1
]}

Dicuonzo, Giordano ^{[1
]}

机构：

[1] Univ Campus Biomed, Dipartimento Sci Biomed, I-00128 Rome, Italy

[2] Univ Roma La Sapienza, Dipartimento Sanita Pubbl, Rome, Italy

来源：

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2009年 / 55卷 / 01期

关键词：

Staphylococcus aureus; coagulase-negative staphylococci; macrolide resistance genes; macrolide resistance phenotypes; PFGE; INDUCIBLE CLINDAMYCIN RESISTANCE; GENES; LINCOSAMIDES; PREVALENCE; GUIDELINES; MECHANISMS; MRSA;

D O I：

10.1111/j.1574-695X.2008.00499.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

One hundred macrolide-resistant staphylococcal isolates from clinically relevant infections in Italy during a 19-month period were studied. Four distinct resistance phenotypes were observed using the triple-disk induction test (erythromycin, clindamycin, telithromycin): the cMLS(B) phenotype (24 isolates); the iMLS(B) phenotype (41 isolates); the MS phenotype (three isolates); and the iMTS phenotype (erythromycin-induced telithromycin resistance) (32 isolates). ermC and ermA genes predominated within erythromycin-resistant Staphylococcus aureus isolates with iMLS(B) phenotype and cMLS(B) phenotype, respectively. Among erythromycin-resistant CoNS isolates, half of the strains showed the iMTS or MS/msrA association, and ermC gene predominated among isolates with MLSB phenotype. By pulsed-field gel electrophoresis, high genetic heterogeneity was observed among the isolates studied. Both independent acquisition of macrolide resistance genes and spread of specific resistant clones were observed. Association between certain clonal types and specific types of infection could be detected. To our knowledge, this is the first report on characterization of erythromycin-resistant staphylococci in Italy.

引用

页码：62 / 67

页数：6

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