Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab

被引:145
作者
Li, H
Dunn, JJ
Luft, BJ
Lawson, CL
机构
[1] BROOKHAVEN NATL LAB,DEPT BIOL,UPTON,NY 11973
[2] SUNY STONY BROOK,SCH MED,DIV INFECT DIS,STONY BROOK,NY 11794
关键词
D O I
10.1073/pnas.94.8.3584
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
OspA (outer surface protein A) is an abundant immunogenic lipoprotein of the Lyme disease spirochete Borrelia burgdorferi. The crystal structure of a soluble recombinant form of OspA was solved in a complex with the Fab fragment of mouse monoclonal antibody 184.1 and refined to a resolution of 1.9 Angstrom. OspA has a repetitive antiparallel beta topology with an unusual nonglobular region of ''freestanding'' sheet connecting globular N- and C-terminal domains. Arrays of residues with alternating charges are a predominant feature of the folding pattern in the nonglobular region. The 184.1 epitope overlaps with a well conserved surface in the N-terminal domain, and a hydrophobic cavity buried in a positively charged cleft in the C-terminal domain is a potential binding site for an unknown ligand. An exposed variable region on the C terminal domain of OspA is predicted to be an important factor in the worldwide effectiveness of OspA-based vaccines.
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页码:3584 / 3589
页数:6
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