Dynamics of the DNA damage response: insights from live-cell imaging

被引：11

作者：

Karanam, Ketki ^{[1
]}

Loewer, Alexander ^{[2
]}

Lahav, Galit ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA USA

[2] Max Delbruek Ctr, Berlin, Germany

来源：

BRIEFINGS IN FUNCTIONAL GENOMICS | 2013年 / 12卷 / 02期

基金：

美国国家卫生研究院;

关键词：

live-cell imaging; single cell; DNA damage; fluorescence microscopy; dynamics; DOUBLE-STRAND BREAKS; FLUORESCENCE CORRELATION SPECTROSCOPY; PROTEIN DYNAMICS; LIVING CELLS; NUCLEAR FOCI; MOBILITY; FRAP; CANCER; SITES; HETEROGENEITY;

D O I：

10.1093/bfgp/els059

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

All organisms have to safeguard the integrity of their genome to prevent malfunctioning and oncogenic transformation. Sophisticated DNA damage response mechanisms have evolved to detect and repair genomic lesions. With the emergence of live-cell microscopy of individual cells, we now begin to appreciate the complex spatiotemporal kinetics of the DNA damage response and can address the causes and consequences of the heterogeneity in the responses of genetically identical cells. Here, we highlight key discoveries where live-cell imaging has provided unprecedented insights into how cells respond to DNA double-strand breaks and discuss the main challenges and promises in using this technique.

引用

页码：109 / 117

页数：9

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