Comprehensive functional analysis of the catalase gene family in Arabidopsis thaliana

被引：214

作者：

Du, Yan-Yan ^{[1
,2
]}

Wang, Peng-Cheng ^{[1
]}

Chen, Jia ^{[2
]}

Song, Chun-Peng ^{[1
]}

机构：

[1] Henan Univ, Lab Plant Stress Biol, Kaifeng 475001, Peoples R China

[2] China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100094, Peoples R China

来源：

JOURNAL OF INTEGRATIVE PLANT BIOLOGY | 2008年 / 50卷 / 10期

基金：

中国国家自然科学基金;

关键词：

catalase; isozymes; reactive oxygen species; stress; transcription;

D O I：

10.1111/j.1744-7909.2008.00741.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Arabidopsis, catalase (CAT) genes encode a small family of proteins including CAT1, CAT2 and CAT3, which catalyze the decomposition of hydrogen peroxide (H2O2) and play an important role in controlling homeostasis of reactive oxygen species (ROS). Here, we analyze the expression profiles and activities of three catalases under different treatments including drought, cold, oxidative stresses, abscisic acid and salicylic acid in Arabidopsis. Our results reveal that CAT1 is an important player in the removal of H2O2 generated under various environmental stresses. CAT2 and CAT3 are major H2O2 scavengers that contribute to ROS homeostasis in light or darkness, respectively. In addition, CAT2 is activated by cold and drought stresses and CAT3 is mainly enhanced by abscisic acid and oxidative treatments as well as at the senescence stage. These results, together with previous data, suggest that the network of transcriptional control explains how CATs and other scavenger enzymes such as peroxidase and superoxide dismutase may be coordinately regulated during development, but differentially expressed in response to different stresses for controlling ROS homeostasis.

引用

页码：1318 / 1326

页数：9

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