A study on the reproductive toxicity of erythrosine in male mice

被引:37
作者
Aziz, AHA
Shouman, SA
Attia, AS
Saad, SF
机构
[1] CAIRO UNIV, DEPT PHARMACOL & TOXICOL, CAIRO, EGYPT
[2] CAIRO UNIV, NATL CANC INST, DEPT CANC BIOL, CAIRO, EGYPT
关键词
erythrosine; spermatogenesis; reproduction; colouring agents;
D O I
10.1006/phrs.1997.0158
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Worldwide usage of different colouring agents in the food industry prompted us to study their toxicity, The potential adverse effects of erythrosine (ER, FD & C Red No.3) on the spermatogenesis process were investigated in adult mice. Testicular lactic dehydrogenase isoenzyme activity (LDH-X), a pachytene spermatocyte marker of testicular toxicity, was significantly decreased to 71.8% and 68.6% of the control value after daily p,o. administration of ER (21 days) in doses of 68 and 136 mg kg(-1) respectively. At the same time, the normal average epididymal sperm count as well as the percentage of motile sperms were significantly inhibited by about 50% and 57% respectively. Moreover, ER was shown to disrupt the normal morphology of the sperm head. Thus, after 5 daily p,o administrations of ER in doses of 680 and 1360 mg kg(-1)(equivalent to 10 and 20% of its LD50) it increased the incidence of sperms with abnormal head by about 57% and 65% respectively. The induced increase in sperm abnormalities could enhance the spermatogenic dysfunction and germ cell mutagenicity. These findings indicate that ER in the used doses has a potential toxic effect on spermatogenesis in mice and in turn, it may affect its testicular function and reproductive performance. (C) 1997 The Italian Pharmacological Society.
引用
收藏
页码:457 / 462
页数:6
相关论文
共 41 条
[31]   SPERM HEAD ABNORMALITY TEST [J].
SCOTT, K ;
TOPHAM, JC .
MUTATION RESEARCH, 1982, 100 (1-4) :345-350
[32]   SELECTED TESTICULAR ENZYMES AS BIOCHEMICAL MARKERS FOR PROCARBAZINE-INDUCED TESTICULAR TOXICITY [J].
SHEN, RS ;
LEE, IP .
ARCHIVES OF TOXICOLOGY, 1984, 55 (04) :233-238
[33]   QUANTITATING THE SYNERGISTIC EFFECT OF SMOKING AND ALCOHOL-CONSUMPTION WITH THE MICRONUCLEUS TEST ON HUMAN BUCCAL MUCOSA CELLS [J].
STICH, HF ;
ROSIN, MP .
INTERNATIONAL JOURNAL OF CANCER, 1983, 31 (03) :305-308
[34]  
TANDON R, 1992, VET HUM TOXICOL, V34, P517
[35]   CHEMICALLY-INDUCED TRANSMISSIBLE ABNORMALITIES IN SPERM-HEAD SHAPE [J].
TOPHAM, JC .
MUTATION RESEARCH, 1980, 70 (01) :109-114
[36]  
Verma P K, 1993, Indian J Med Sci, V47, P204
[37]  
WHEAT TE, 1983, ISOZYMES-CURR T BIOL, V7, P113
[38]  
WHORTON MD, 1982, W J MED, V137, P1521
[39]   AN EVALUATION OF THE MOUSE SPERM MORPHOLOGY TEST AND OTHER SPERM TESTS IN NON-HUMAN MAMMALS - A REPORT OF THE UNITED-STATES-ENVIRONMENTAL-PROTECTION-AGENCY GENE-TOX PROGRAM [J].
WYROBEK, AJ ;
GORDON, LA ;
BURKHART, JG ;
FRANCIS, MW ;
KAPP, RW ;
LETZ, G ;
MALLING, HV ;
TOPHAM, JC ;
WHORTON, MD .
MUTATION RESEARCH, 1983, 115 (01) :1-72
[40]  
WYROBEK AJ, 1978, CHEM MUTAGENS PRINCI, V5, P261