HSDL2 Promotes Bladder Cancer Growth In Vitro and In Vivo

被引：11

作者：

Jia, Ling-Hua ^{[1
,2
]}

Hu, Mei-Di ^{[3
]}

Liu, Yuan ^{[4
]}

Xiong, Xing ^{[2
]}

Wang, Wei-Jia ^{[5
]}

Wang, Jin-Gen ^{[2
]}

Li, Qiu-Gen ^{[6
]}

机构：

[1] Nanchang Univ, Jiangxi Med Coll, Grad Fac, Nanchang 330006, Jiangxi, Peoples R China

[2] Nanchang Univ, Jiangxi Prov Peoples Hosp, Dept Urol, Nanchang 330006, Jiangxi, Peoples R China

[3] Nanchang Univ, Affiliated Hosp 1, Dept Gerontol, Nanchang 330006, Jiangxi, Peoples R China

[4] Fudan Univ, Peoples Hosp Shanghai 5, Div Nephrol, Shanghai 200240, Peoples R China

[5] Nanchang Univ, Affiliated Hosp 1, Dept Pathol, Nanchang 330006, Jiangxi, Peoples R China

[6] Nanchang Univ, Jiangxi Prov Peoples Hosp, Dept Resp Med, Nanchang 330006, Jiangxi, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MEDICAL SCIENCES | 2019年 / 16卷 / 05期

关键词：

bladder cancer; HSDL2; shRNA; cell proliferation; apoptosis;

D O I：

10.7150/ijms.31288

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Bladder cancer is a common malignant urinary tumor, and patients with bladder cancer have poor prognosis. Abnormal lipid metabolism in peroxisomes is involved in tumor progression. Hydroxysteroid dehydrogenase-like 2 (HSDL2) localized in peroxisomes regulates fatty acid synthesis. In the present study, we reported that HSDL2 was upregulated in two human bladder cancer cell lines 5637 and T24 compared to normal human urothelial cells. Furthermore, lentiviral-mediated HSDL2 knockdown inhibited the proliferation and colony formation while promoted the apoptosis of human bladder cancer T24 cells in vitro. In nude mice HSDL2 knockdown inhibited the growth of T24 derived xenografts in vivo. In conclusion, our results suggest that HSDL2 plays an oncogenic role in bladder cancer and might serve as a potential target for bladder cancer therapy.

引用

页码：654 / 659

页数：6

共 17 条

[1] ETHER-LINKED GLYCEROLIPIDS IN HUMAN-BRAIN TUMORS [J].

ALBERT, DH ;

ANDERSON, CE .

LIPIDS, 1977, 12 (02) :188-192

[2] Ether lipid generating enzyme AGPS alters the balance of structural and signaling lipids to fuel cancer pathogenicity [J].

Benjamin, Daniel I. ;

Cozzo, Alyssa ;

Ji, Xiaodan ;

Roberts, Lindsay S. ;

Louie, Sharon M. ;

Mulvihill, Melinda M. ;

Luo, Kunxin ;

Nomura, Daniel K. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (37) :14912-14917

[3] Impaired neuronal migration and endochondral ossification in Pex7 knockout mice:: a model for rhizomelic chondrodysplasia punctata [J].

Brites, P ;

Motley, AM ;

Gressens, P ;

Mooyer, PAW ;

Ploegaert, I ;

Everts, V ;

Evrard, P ;

Carmeliet, P ;

Dewerchin, M ;

Schoonjans, L ;

Duran, M ;

Waterham, HR ;

Wanders, RJA ;

Baes, M .

HUMAN MOLECULAR GENETICS, 2003, 12 (18) :2255-2267

[4] Lentivirus-mediated silencing of HSDL2 suppresses cell proliferation in human gliomas [J].

Chen Ruokun ;

Xue Yake ;

Yang Fengdong ;

Wei Xinting ;

Song Laijun ;

Liu Xianzhi .

TUMOR BIOLOGY, 2016, 37 (11) :15065-15077

[5] Molecular cloning and characterization of a novel human hydroxysteroid dehydrogenase-like 2 (HSDL2) cDNA from fetal brain [J].

Dai, JF ;

Xie, Y ;

Wu, QH ;

Wang, L ;

Yin, G ;

Ye, X ;

Zeng, L ;

Xu, J ;

Ji, CN ;

Gu, SH ;

Huang, QS ;

Zhao, RCH ;

Mao, YM .

BIOCHEMICAL GENETICS, 2003, 41 (5-6) :165-174

[6] Structural and functional roles of ether lipids [J].

Dean, John M. ;

Lodhi, Irfan J. .

PROTEIN & CELL, 2018, 9 (02) :196-206

[7] The Proteome of Human Liver Peroxisomes: Identification of Five New Peroxisomal Constituents by a Label-Free Quantitative Proteomics Survey [J].

Gronemeyer, Thomas ;

Wiese, Sebastian ;

Ofman, Rob ;

Bunse, Christian ;

Pawlas, Magdalena ;

Hayen, Heiko ;

Eisenacher, Martin ;

Stephan, Christian ;

Meyer, Helmut E. ;

Waterham, Hans R. ;

Erdmann, Ralf ;

Wanders, Ronald J. ;

Warscheid, Bettina .

PLOS ONE, 2013, 8 (02)

[8] In search for function of two human orphan SDR enzymes: Hydroxysteroid dehydrogenase like 2 (HSDL2) and short-chain dehydrogenase/reductase-orphan (SDR-O) [J].

Kowalik, Dorota ;

Haller, Ferdinand ;

Adamski, Jerzy ;

Moeller, Gabriele .

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2009, 117 (4-5) :117-124

[9]

Liu M, 2017, BIOCELL, V41, P19

[10] Peroxisomes: A Nexus for Lipid Metabolism and Cellular Signaling [J].

Lodhi, Irfan J. ;

Semenkovich, Clay F. .

CELL METABOLISM, 2014, 19 (03) :380-392

← 1 2 →