FULL-LENGTH VP2 GENE ANALYSIS OF CANINE PARVOVIRUS REVEALS EMERGENCE OF NEWER VARIANTS IN INDIA

被引:11
作者
Nookala, Mangadevi [1 ]
Mukhopadhyay, Hirak Kumar [1 ]
Sivaprakasam, Amsaveni [1 ]
Balasubramanian, Brindhalakshmi [1 ]
Antony, Prabhakar Xavier [1 ]
Thanislass, Jacob [2 ]
Srinivas, Mouttou Vivek [1 ]
Pillai, Raghavan Madhusoodanan [1 ]
机构
[1] Rajiv Gandhi Inst Vet Educ & Res, Dept Vet Microbiol, Pondicherry, India
[2] Rajiv Gandhi Inst Vet Educ & Res, Dept Vet Biochem, Pondicherry, India
关键词
canine parvovirus (CPV); mutation; phylogenetic analysis; polymerase chain reaction (PCR); sequencing; MINK ENTERITIS VIRUS; HOST-RANGE; MOLECULAR CHARACTERIZATION; FIELD STRAINS; EVOLUTION; FELINE; VACCINE; RECOMBINATION; EPIDEMIOLOGY; BRAZIL;
D O I
10.1556/030.63.2016.010
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The canine parvovirus (CPV) infection is a highly contagious and serious enteric disease of dogs with high fatality rate. The present study was taken up to characterize the full-length viral polypeptide 2 (VP2) gene of CPV of Indian origin along with the commercially available vaccines. The faecal samples from parvovirus suspected dogs were collected from various states of India for screening by PCR assay and 66.29% of samples were found positive. Six CPV-2a, three CPV-2b, and one CPV-2c types were identified by sequence analysis. Several unique and existing mutations have been noticed in CPV types analyzed indicating emergence of newer variants of CPV in India. The phylogenetic analysis revealed that all the field CPV types were grouped in different subclades within two main clades, but away from the commercial vaccine strains. CPV-2b and CPV-2c types with unique mutations were found to be establishing in India apart from the prevailing CPV-2a type. Mutations and the positive selection of the mutants were found to be the major mechanism of emergence and evolution of parvovirus. Therefore, the incorporation of local strain in the vaccine formulation may be considered for effective control of CPV infections in India.
引用
收藏
页码:411 / 426
页数:16
相关论文
共 53 条
[1]   THE RECOGNITION OF PARVOVIRUS CAPSIDS BY ANTIBODIES [J].
AGBANDJE, M ;
PARRISH, CR ;
ROSSMANN, MG .
SEMINARS IN VIROLOGY, 1995, 6 (04) :219-231
[2]   High local genetic diversity of canine parvovirus from Ecuador [J].
Aldaz, Jaime ;
Garcia-Diaz, Juan ;
Calleros, Lucia ;
Sosa, Katia ;
Iraola, Gregorio ;
Marandino, Ana ;
Hernandez, Martin ;
Panzera, Yanina ;
Perez, Ruben .
VETERINARY MICROBIOLOGY, 2013, 166 (1-2) :214-219
[3]   Genetic analysis of canine parvovirus isolates (CPV-2) from dogs in Italy [J].
Battilani, M ;
Ciulli, S ;
Tisato, E ;
Prosperi, S .
VIRUS RESEARCH, 2002, 83 (1-2) :149-157
[4]   Evidence for evolution of canine parvovirus type 2 in Italy [J].
Buonavoglia, C ;
Martella, V ;
Pratelli, A ;
Tempesta, M ;
Cavalli, A ;
Buonavoglia, D ;
Bozzo, G ;
Elia, G ;
Decaro, N ;
Carmichael, L .
JOURNAL OF GENERAL VIROLOGY, 2001, 82 :3021-3025
[5]  
Castro TX, 2010, BRAZ J MICROBIOL, V41, P1093, DOI [10.1590/S1517-83822010000400031, 10.1590/S1517-838220100004000031]
[6]   Detection and genetic characterization of Canine parvovirus and Canine coronavirus strains circulating in district of Tirana in Albania [J].
Cavalli, Alessandra ;
Desario, Costantina ;
Kusi, Ilir ;
Mari, Viviana ;
Lorusso, Eleonora ;
Cirone, Francesco ;
Kumbe, Ilirjan ;
Colaianni, Maria Loredana ;
Buonavoglia, Domenico ;
Decaro, Nicola .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2014, 26 (04) :563-566
[7]   Canine parvovirus in asymptomatic feline carriers [J].
Clegg, S. R. ;
Coyne, K. P. ;
Dawson, S. ;
Spibey, N. ;
Gaskell, R. M. ;
Radford, A. D. .
VETERINARY MICROBIOLOGY, 2012, 157 (1-2) :78-85
[8]   Analysis of the full-length VP2 protein of canine parvoviruses circulating in Hungary [J].
Csagola, Attila ;
Varga, Szilvia ;
Lorincz, Marta ;
Tuboly, Tamas .
ARCHIVES OF VIROLOGY, 2014, 159 (09) :2441-2444
[9]  
Decaro N, 2004, MICROBIOLOGICA, V27, P375
[10]   Genetic analysis of canine parvovirus type 2c [J].
Decaro, Nicola ;
Desario, Costantina ;
Parisi, Antonio ;
Martella, Vito ;
Lorusso, Alessio ;
Miccolupo, Angela ;
Mari, Viviana ;
Colaianni, Maria Loredana ;
Cavalli, Alessandra ;
Di Trani, Livia ;
Buonavoglia, Canio .
VIROLOGY, 2009, 385 (01) :5-10