Unveiling the anticancer and antimycobacterial potentials of bioengineered gold nanoparticles

被引：34
作者：
Govindaraju, K. [1 ]
Vasantharaja, R. [1 ]
Suganya, K. S. Uma [1 ]
Anbarasu, S. [2 ]
Revathy, K. [2 ]
Pugazhendhi, A. [3 ]
Karthickeyan, D. [4 ]
Singaravelu, G. [5 ]
机构：
[1] Sathyabama Inst Sci & Technol, Ctr Ocean Res DST FIST Sponsored Ctr, Chennai 600119, Tamil Nadu, India
[2] Sathyabama Inst Sci & Technol, Ctr Drug Discovery & Dev, Chennai 600119, Tamil Nadu, India
[3] Ton Duc Thang Univ, Fac Environm & Labour Safety, Innovat Green Prod Synth & Renewable Environm Dev, Ho Chi Minh City, Vietnam
[4] Govt Coll Engn, Dept Phys, Bargur 635104, India
[5] Thiruvalluvar Univ, Dept Zool, Vellore 632115, Tamil Nadu, India
来源：
PROCESS BIOCHEMISTRY | 2020年 / 96卷
关键词：
Gold nanoparticles; Anticancer; Toxicity; Antimycobacterial activity; Pongammia pinnata; SILVER NANOPARTICLES; BIOGENIC SYNTHESIS; GREEN SYNTHESIS; CANCER; APOPTOSIS; ASSAY;
D O I：
10.1016/j.procbio.2020.06.016
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Synthesis of gold nanoparticles was carried out using Pongammia pinnata (pongam) leaf extract and their anticancer and antimycobacterial activities were studied. Gold nanoparticle formation was confirmed by UV-vis, XRD and HR-TEM. The anticancer efficacies of the biogenic gold nanoparticles were analyzed using cytotoxicity, cell morphology analysis, oxidative DNA damage, apoptosis detection and toxicity studies. Biogenic gold nanoparticles inhibited breast cancer cell line (MCF-7) proliferation with an efficacy of IC50 of 1.85 mu g/mL. The antimycobacterial potential of the biogenic gold nanoparticles was screened against M. tuberculosis by Luciferase Reporter Phage (LRP) assay. The gold nanoparticles showed inhibition against sensitive M. tuberculosis with the minimum inhibitory concentration (MIC) of 10 mu g/mL whereas no inhibition was found against the rifampicin resistant M. tuberculosis.
引用
收藏
页码：213 / 219
页数：7
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