Bone Marrow-Derived Mesenchymal Stem Cells Maintain the Resting Phenotype of Microglia and Inhibit Microglial Activation

被引：50

作者：

Yan, Ke ^{[1
]}

Zhang, Run ^{[1
]}

Sun, Chengmei ^{[1
]}

Chen, Lei ^{[1
,2
]}

Li, Peng ^{[1
,3
]}

Liu, Yi ^{[1
]}

Peng, Lingmei ^{[1
,4
]}

Sun, Haitao ^{[1
]}

Qin, Kun ^{[1
,5
]}

Chen, Fanfan ^{[1
,3
]}

Huang, Weiyi ^{[1
]}

Chen, Yuxin ^{[1
]}

Lv, Bingke ^{[1
]}

Du, Mouxuan ^{[1
]}

Zou, Yuxi ^{[1
]}

Cai, Yingqian ^{[1
]}

Qin, Lingsha ^{[1
]}

Tang, Yanping ^{[1
]}

Jiang, Xiaodan ^{[1
]}

机构：

[1] Southern Med Univ, Guangdong Prov Key Lab Brain Funct Repair & Regen, Neurosurg Inst Guangdong Prov, Natl Key Clin Specialty,Dept Neurosurg,Zhujian Ho, Guangzhou, Guangdong, Peoples R China

[2] Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Dept Neurosurg, Shenzhen, Peoples R China

[3] Guangzhou First Peoples Hosp, Dept Neurosurg, Guangzhou, Guangdong, Peoples R China

[4] First Peoples Hosp Foshan, Dept Neurol, Foshan, Peoples R China

[5] Guangdong Acad Med Sci, Guangdong Gen Hosp, Dept Neurosurg, Guangzhou, Guangdong, Peoples R China

来源：

PLOS ONE | 2013年 / 8卷 / 12期

关键词：

STROMAL CELLS; NITRIC-OXIDE; PROLIFERATION; BRAIN; INTERLEUKIN-10; PHAGOCYTOSIS; PROTECTION; NEURONS; MODEL;

D O I：

10.1371/journal.pone.0084116

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Many studies have shown that microglia in the activated state may be neurotoxic. It has been proven that uncontrolled or over-activated microglia play an important role in many neurodegenerative disorders. Bone marrow-derived mesenchymal stem cells (BMSCs) have been shown in many animal models to have a therapeutic effect on neural damage. Such a therapeutic effect is attributed to the fact that BMSCs have the ability to differentiate into neurons and to produce trophic factors, but there is little information available in the literature concerning whether BMSCs play a therapeutic role by affecting microglial activity. In this study, we triggered an inflammatory response situation in vitro by stimulating microglia with the bacterial endotoxin lipopolysaccharide (LPS), and then culturing these microglia with BMSC-conditioned medium (BMSC-CM). We found that BMSC-CM significantly inhibited proliferation and secretion of pro-inflammatory factors by activated microglia. Furthermore, we found that the phagocytic capacity of microglia was also inhibited by BMSC-CM. Finally, we investigated whether the induction of apoptosis and the production of nitric oxide (NO) were involved in the inhibition of microglial activation. We found that BMSC-CM significantly induced apoptosis of microglia, while no apoptosis was apparent in the LPS-stimulated microglia. Our study also provides evidence that NO participates in the inhibitory effect of BMSCs. Our experimental results provide evidence that BMSCs have the ability to maintain the resting phenotype of microglia or to control microglial activation through their production of several factors, indicating that BMSCs could be a promising therapeutic tool for treatment of diseases associated with microglial activation.

引用

页数：8

共 42 条

[1] Bone marrow mesenchymal progenitor cells inhibit lymphocyte proliferation by activation of the programmed death 1 pathway [J].