Comparative study of four extraction methods for enterovirus recovery from wastewater and sewage sludge

被引：14

作者：

Belguith, K

Hassen, A

Aouni, M

机构：

[1] Inst Natl Rech Sci & Tech, Lab Eau & Environm, Tunis, Tunisia

[2] Fac Pharm Monastir, Lab Maladies Transmissibles & Substances Biol Act, Monastir, Tunisia

来源：

BIORESOURCE TECHNOLOGY | 2006年 / 97卷 / 03期

关键词：

enteroviruses; wastewater; sludge; extraction method; cell culture; PCR;

D O I：

10.1016/j.biortech.2005.03.022

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

This study investigated four methods for the recovery of enteroviruses from sterilized raw wastewater, activated sludge, thickened sludge and treated wastewater, inoculated with Echovirus 11, Gregory prototype. The adsorption-elution method recommended by the US Environmental Protection Agency (EPA) was better for Echovirus 11 recovery than a sonication method, a modified EPA method and a membrane adsorption elution method since it resulted in the highest detection levels by cell culture and RT-PCR (Friedman's test, p < 0.00041 and p = 0.041, respectively). (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：414 / 419

页数：6

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