Regulation of Spine Density and Morphology by IQGAP1 Protein Domains

被引：16

作者：

Jausoro, Ignacio ^{[1
,2
]}

Mestres, Ivan ^{[1
,2
]}

Quassollo, Gonzalo ^{[1
,2
]}

Masseroni, Lujan ^{[1
,2
]}

Heredia, Florencia ^{[1
,2
]}

Caceres, Alfredo ^{[1
,2
]}

机构：

[1] INIMEC CONICET, Inst Mercedes & Martin Ferreyra, Neurobiol Lab, Cordoba, Argentina

[2] Univ Nacl Cordoba, RA-5000 Cordoba, Argentina

来源：

PLOS ONE | 2013年 / 8卷 / 02期

关键词：

DENDRITIC SPINES; CELL POLARIZATION; ARP2/3; COMPLEX; SMALL GTPASES; ACTIN; CDC42; DYNAMICS; ROLES; RAC1; RHO;

D O I：

10.1371/journal.pone.0056574

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

IQGAP1 is a scaffolding protein that regulates spine number. We now show a differential role for IQGAP1 domains in spine morphogenesis, in which a region of the N-terminus that promotes Arp2/3-mediated actin polymerization and branching stimulates spine head formation while a region that binds to Cdc42 and Rac is required for stalk extension. Conversely, IQGAP1 rescues spine deficiency induced by expression of dominant negative Cdc42 by stimulating formation of stubby spines. Together, our observations place IQGAP1 as a crucial regulator of spine number and shape acting through the N-Wasp Arp2/3 complex, as well as upstream and downstream of Cdc42.

引用

页数：10

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