Effects of 50 Hz electromagnetic fields on voltage-gated Ca2+ channels and their role in modulation of neuroendocrine cell proliferation and death

被引:180
作者
Grassi, C
D'Ascenzo, M
Torsello, A
Martinotti, G
Wolf, F
Cittadini, A
Azzena, GB
机构
[1] Univ Sacred Heart, Sch Med, Inst Human Physiol, I-00168 Rome, Italy
[2] Univ Sacred Heart, Sch Med, Inst Gen Pathol, I-00168 Rome, Italy
关键词
extremely low-frequency electromagnetic fields; calcium channels; cell proliferation; apoptosis; channel expression; neuroblastoma; rat pituitary cells;
D O I
10.1016/j.ceca.2003.09.001
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Possible correlation between the effects of electromagnetic fields (EFs) on voltage-gated Ca2+ channels, cell proliferation and apoptosis was investigated in neural and neurcendocrine cells. Exposure to 50 Hz EFs significantly enhanced proliferation in human neuroblastoma IMR32 (+40%) and rat pituitary GH3 cells (+38%). In IMR32 cells EF stimulation also inhibited puromycin- and H2O2-induced apoptosis (-22 and -33%, respectively). EF effects on proliferation and apoptosis were counteracted by Ca2+ channel blockade. In whole-cell patch-clamp experiments 24-72 h exposure to EFs increased macroscopic Ba2+-current density in both GH3 (+67%) and IMR32 cells (+40%). Single-channel recordings showed that gating of L and N channels was instead unaffected, thus suggesting that the observed enhancement of current density was due to increased number of voltage-gated Ca2+ channels. Western blot analysis of plasma membrane-enriched microsomal fractions of GH3 and IMR32 cells confirmed enhanced expression of Ca2+ channel subunit alpha(1) following exposure to EFs. These data provide the first direct evidence that EFs enhance the expression of voltage-gated Ca2+ channels on plasma membrane of the exposed cells. The consequent increase in Ca2+ influx is likely responsible for the EF-induced modulation of neuronal cell proliferation and apoptosis. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:307 / 315
页数:9
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