Thioredoxin reductase reduces lipid hydroperoxides and spares α-tocopherol

被引：24

作者：

May, JM ^{[1
]}

Morrow, JD ^{[1
]}

Burk, RF ^{[1
]}

机构：

[1] Vanderbilt Univ, Sch Med, Dept Med, Nashville, TN 37232 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 292卷 / 01期

关键词：

lipid hydroperoxides; selenium; thioredoxin reductase; thioredoxin; ferricyanide; alpha-tocopherol; human erythrocytes;

D O I：

10.1006/bbrc.2002.6617

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated whether and how rat liver thioredoxin reductase spares alpha-tocopherol in biomembranes. Purified hydroperoxides of beta-linoleoyl-gamma-palmitoylphosphatidylcholine were decreased 35% by treatment with thioredoxin reductase and 54% by thioredoxin reductase plus E. coli thioredoxin. Thioredoxin reductase also halved the amount of hydroperoxides that had been formed during photoperoxidation of liposomes composed of beta-linoleoyl-gamma-palmitoylphosphatidylcholine, and of emulsions of both cholesterol and cholesteryl linolenate. In erythrocyte ghosts, thioredoxin reductase spared alpha-tocopherol from oxidation by both soybean lipoxygenase and ferricyanide. Thioredoxin reductase also decreased F-2-isoprostanes in ghosts oxidized by ferricyanide, suggesting that its ability to spare alpha-tocopherol relates to reduction of lipid hydroperoxides. (C) 2002 Elsevier Science (USA).

引用

页码：45 / 49

页数：5

共 31 条

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