The growth of filaments under macromolecular confinement using scaling theory

被引：3

作者：

Zhu, Lin ^{[1
,2
]}

Pan, Wei ^{[1
,2
]}

Lu, Xi ^{[3
]}

Li, Desheng ^{[3
]}

Zhao, Jiang ^{[3
]}

Liang, Dehai ^{[1
,2
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China

[2] Peking Univ, Coll Chem & Mol Engn, Key Lab Polymer Chem & Phys, Minist Educ, Beijing 100871, Peoples R China

[3] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 88期

基金：

中国国家自然科学基金;

关键词：

CROWDING STABILIZES; AGGREGATION; KINETICS; POLYMERIZATION; APOMYOGLOBIN; SEPARATION; DYNAMICS; RESPECT; COLD;

D O I：

10.1039/c5cc06748k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Quantitatively describing macromolecular confinement is still a challenge. Using the assembly of DNA tiles in a polyacrylamide network as a model, we studied the effect of macromolecular confinement on the growth of the filament by scaling theory. The results show that the confinement regulates the morphology, the initial growth rate v, and the eventual length of the filament N-m. The initial growth rate is dependent on the medium viscosity eta as nu alpha eta(-0.94), and the filament adjusts its length in the given confined space as N-mp alpha(xi/R-g)(1.8), with xi being the mesh size of the polyacrylamide solution and R-g being the radius of gyration of polyacrylamide.

引用

页码：15928 / 15931

页数：4

共 40 条

[1] Impact of macromolecular crowding on DNA replication
Akabayov, Barak
Akabayov, Sabine R.
Lee, Seung-Joo
Wagner, Gerhard
Richardson, Charles C.
[J]. NATURE COMMUNICATIONS, 2013, 4
[2] Analytical Ultracentrifugation Studies of Phage φ29 Protein p6 Binding to DNA
Alcorlo, Martin
Jimenez, Mercedes
Ortega, Alvaro
Hermoso, Jose M.
Salas, Margarita
Minton, Allen P.
Rivas, German
[J]. JOURNAL OF MOLECULAR BIOLOGY, 2009, 385 (05) : 1616 - 1629
[3] The crowd you're in with: Effects of different types of crowding agents on protein aggregation
Breydo, Leonid
Reddy, Krishna D.
Piai, Alessandro
Felli, Isabella C.
Pierattelli, Roberta
Uversky, Vladimir N.
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2014, 1844 (02): : 346 - 357
[4] Doi M., 1988, The theory of polymer dynamics.
[5] Joining and scission in the self-assembly of nanotubes from DNA tiles
Ekani-Nkodo, A
Kumar, A
Fygenson, DK
[J]. PHYSICAL REVIEW LETTERS, 2004, 93 (26)
[6] Cell biology - Join the crowd
Ellis, RJ
Minton, AP
[J]. NATURE, 2003, 425 (6953) : 27 - 28
[7] Flory P J., PRINCIPLES POLYM CHE
[8] Gaharwar B, 2015, PROTEIN PEPTIDE LETT, V22, P87
[9] A matrix for DNA separation: Genotyping and sequencing using poly(vinylpyrrolidone) solution in uncoated capillaries
Gao, QF
Yeung, ES
[J]. ANALYTICAL CHEMISTRY, 1998, 70 (07) : 1382 - 1388
[10] Thermodynamics and kinetics of DNA nanotube polymerization from single-filament measurements
Hariadi, Rizal F.
Yurke, Bernard
Winfree, Erik
[J]. CHEMICAL SCIENCE, 2015, 6 (04) : 2252 - 2267

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