Poly(alizarin red)/Graphene modified glassy carbon electrode for simultaneous determination of purine and pyrimidine

被引：43

作者：

Ba, Xi ^{[1
]}

Luo, Liqiang ^{[1
]}

Ding, Yaping ^{[1
]}

Zhang, Zhen ^{[1
]}

Chu, Yuliang ^{[2
]}

Wang, Bijun ^{[1
]}

Ouyang, Xiaoqian ^{[1
]}

机构：

[1] Shanghai Univ, Dept Chem, Shanghai 200444, Peoples R China

[2] Shanghai Univ, Instrumental Anal & Res Ctr, Shanghai 200444, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2012年 / 752卷

基金：

中国国家自然科学基金;

关键词：

Poly alizarin red; Graphene; Purine; Pyrimidine; DOPED DIAMOND ELECTRODES; ALIZARIN RED S; ELECTROCHEMICAL OXIDATION; LIQUID-CHROMATOGRAPHY; DNA; ADENINE; GUANINE; NANOTUBES; GRAPHENE; BASES;

D O I：

10.1016/j.aca.2012.09.031

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, a poly(alizarin red)/Graphene composite film modified glassy carbon electrode (PAR/Graphene/GCE) was prepared for simultaneous determination of four DNA bases (guanine, adenine, thymine and cytosine) without any pretreatment. The morphology and interface property of PAR/Graphene films were examined by scanning electron microscopy and electrochemical impedance spectroscopy. The PAR/Graphene/GCE exhibited excellent electrocatalytic activity toward purine (guanine and adenine) and pyrimidine (thymine and cytosine) in 0.1 M phosphate buffer solution (pH 7.4). Under optimum conditions, differential pulse voltammetry was used to detect the oxidation of purine and pyrimidine. The results showed that PAR/Graphene/GCE exhibited well-separated peaks, low detection limit, high sensitivity and wide linear range for simultaneous detection of purine and pyrimidine. The proposed sensor also has good stability and reproducibility. Furthermore, the modified electrode was applied for the detection of DNA bases in a fish sperm DNA sample with satisfactory results. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.

引用

页码：94 / 100

页数：7

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