Serum protein pattern during cow pregnancy: Acute-phase proteins increase in the peripartum period

被引:41
作者
Cairoli, Fausto
Battocchio, Massimiliano
Veronesi, Maria Cristina
Brambilla, Daniela
Conserva, Francesca
Eberini, Ivano
Wait, Robin
Gianazza, Elisabetta
机构
[1] Univ Milan, Grp Studio Proteom & Struttura Prot, Dipartimento Sci Farmacol, I-20133 Milan, Italy
[2] Univ Milan, Fac Med Vet, Dipartimento Sci Clin Vet, Sez Clin Ostet & Ginecol Vet, Milan, Italy
[3] Univ Milan, Dipartimento Sci Farmacol, Grp Studio Proteom & Struttura Prot, Milan, Italy
[4] Univ London Imperial Coll Sci Technol & Med, Fac Med, Kennedy Inst, Div Rheumatol, London, England
关键词
endometritis; haptoglobin; orosomucoid; peripartum; pregnancy;
D O I
10.1002/elps.200500742
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Serum collected in a time-course mode during the pregnancy of a group of heifers was analyzed by 2-DE under various experimental conditions to optimize resolution of all protein spots. Changes in the levels of some components were detected during the last phase of pregnancy and early postpartum. These included a decrease Of alpha(2)-HS-glycoprotein, an increase of alpha(1)-antichymotrypsin and, with a much larger and more abrupt variation, of orosomucoid and haptoglobin. These findings associate the weeks preceding calving with an acute-phase reaction. Analysis of individual animal's sera by 1-DE demonstrated a higher level of orosomucoid in the sera of cows developing postpartum endometritis during the 2 wk after calving (i.e., in the course of the infection) but a lower level during the 2 wk before calving. This observation could represent an important tool for the prepartum detection of animals prone to develop postpartum endometritis and lead to a more accurate peripartum management of those animals.
引用
收藏
页码:1617 / 1625
页数:9
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