Gas chromatographic method for analysis of conjugated linoleic acids isomers (c9t11, t10c12, and t9t11) in broth media as application in probiotic studies

被引：6

作者：

Alonso, L

Cuesta, EP

Gilliland, SE

机构：

[1] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA

[2] Oklahoma State Univ, Food & Agr Prod Res & Technol Ctr, Stillwater, OK 74078 USA

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2004年 / 42卷 / 03期

关键词：

D O I：

10.1093/chromsci/42.3.167

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A gas chromatography (GC) procedure is assayed for analysis of conjugated linoleic acid (CLA) isomers cis-9, trans-11-octadecadienoic (c9t11); trans-10, 12 cis-octadecadienoic (t10c12); and trans-9, trans-11-octadecadienoic (t9t11) in culture broth by GC using NaOH-BF3 in methanol for methylating and a long capillary (100 m) high-polarity column. Repeatability of the method is assessed; the coefficient of variation for CIA isomers ranges from 4.62 for c9t11 to 8.19 for t9t11. Recovery ranges between 88.01 and 89.76, with a mean value of 89.06 for all CLA isomers studied. This method may be considered advantageous for analysis of CLA isomers in probiotics cultures samples.

引用

页码：167 / 170

页数：4

共 26 条

[1] Fatty acid composition of Spanish shortenings with special emphasis on trans unsaturation content as determined by Fourier transform infrared spectroscopy and gas chromatography [J].