Odd-numbered very-long-chain polyunsaturated fatty acids from the dinoflagellate Amphidinium carterae identified by atmospheric pressure chemical ionization liquid chromatography-mass spectrometry

被引：18

作者：

Rezanka, Tomas ^{[1
]}

Nedbalova, Linda ^{[2
,3
]}

Sigler, Karel ^{[1
]}

机构：

[1] Acad Sci Czech Republic, Inst Microbiol, CR-14220 Prague, Czech Republic

[2] Charles Univ Prague, Fac Sci, Dept Ecol, CR-12844 Prague 2, Czech Republic

[3] Acad Sci Czech Republic, Inst Bot, CS-37982 Trebon, Czech Republic

来源：

PHYTOCHEMISTRY | 2008年 / 69卷 / 16期

关键词：

Amphidinium carterae; Marine dinoflagellate; Odd very-long-chain polyunsaturated fatty acids; Liquid chromatography-mass spectrometry-atmospheric pressure chemical ionization;

D O I：

10.1016/j.phytochem.2008.08.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the enrichment of odd very-long-chain polyunsaturated fatty acids (VLCPUFAs) by means of RP-HPLC and argentation TLC from total fatty acids of the dinoflagellate A. carterae and their identification as picolinyl esters by means of microbore liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization (LC-MS/APCI). The combination of argentation TLC and LC-MS/ APCI was used to identify rare and unusual odd VLCPUFAs up to nonacosahexaenoic acid. Two acids, (allZ)-nonacosa-11,14,17,20,23-pentaenoic acid (29:5n-6) and (allZ)-nonacosa-11,14,17,20,23,26-hexaenoic acid (29:6n-3), were synthesized for the first time to unambiguously confirm their structure. Possible biosynthetic pathways for odd VLCPUFAs are also proposed. All rights reserved (C) 2008 Elsevier Ltd..

引用

页码：2849 / 2855

页数：7

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