Odd-numbered very-long-chain polyunsaturated fatty acids from the dinoflagellate Amphidinium carterae identified by atmospheric pressure chemical ionization liquid chromatography-mass spectrometry

被引:18
作者
Rezanka, Tomas [1 ]
Nedbalova, Linda [2 ,3 ]
Sigler, Karel [1 ]
机构
[1] Acad Sci Czech Republic, Inst Microbiol, CR-14220 Prague, Czech Republic
[2] Charles Univ Prague, Fac Sci, Dept Ecol, CR-12844 Prague 2, Czech Republic
[3] Acad Sci Czech Republic, Inst Bot, CS-37982 Trebon, Czech Republic
关键词
Amphidinium carterae; Marine dinoflagellate; Odd very-long-chain polyunsaturated fatty acids; Liquid chromatography-mass spectrometry-atmospheric pressure chemical ionization;
D O I
10.1016/j.phytochem.2008.08.021
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A method is described for the enrichment of odd very-long-chain polyunsaturated fatty acids (VLCPUFAs) by means of RP-HPLC and argentation TLC from total fatty acids of the dinoflagellate A. carterae and their identification as picolinyl esters by means of microbore liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization (LC-MS/APCI). The combination of argentation TLC and LC-MS/ APCI was used to identify rare and unusual odd VLCPUFAs up to nonacosahexaenoic acid. Two acids, (allZ)-nonacosa-11,14,17,20,23-pentaenoic acid (29:5n-6) and (allZ)-nonacosa-11,14,17,20,23,26-hexaenoic acid (29:6n-3), were synthesized for the first time to unambiguously confirm their structure. Possible biosynthetic pathways for odd VLCPUFAs are also proposed. All rights reserved (C) 2008 Elsevier Ltd..
引用
收藏
页码:2849 / 2855
页数:7
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