Molecular cloning and sequence analysis of an endo-inulinase gene from Arthrobacter sp.

被引：26
作者：
Kang, SI
Kim, SI [1 ]
机构：
[1] Seoul Natl Univ, Coll Agr & Life Sci, Dept Agr Chem, Suwon 441744, South Korea
[2] Seoul Natl Univ, Coll Agr & Life Sci, Res Ctr New Biomat Agr, Suwon 441744, South Korea
来源：
BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 07期
关键词：
Arthrobacter sp; beta-fructosidase motif; catalytic residue; endo-inulinase; gene cloning;
D O I：
10.1023/A:1005514603287
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A 3.0-kb DNA fragment containing an endo-inulinase gene was cloned from Arthrobacter sp. S37. It contained a single open reading frame of 2439 bp, encoding a polypeptide composed of signal peptide of 53 amino acids and mature protein of 759 amino acids. From the comparison with amino acids sequences of fructan hydrolases and invertase, five highly conserved regions including the beta-fructosidase motif were found. The sequence of the endo-inulinase had the identity in the range of 13.3% to 16.0%.
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页码：569 / 574
页数：6
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