Purification and characterization of prophenoloxidase from Galleria mellonella L.

被引：23

作者：

Demir, Dudu ^{[1
]}

Gencer, Nahit ^{[1
]}

Er, Aylin ^{[2
]}

机构：

[1] Balikesir Univ, Sci & Art Fac, Dept Chem, TR-10100 Balikesir, Turkey

[2] Balikesir Univ, Sci & Art Fac, Dept Biol, TR-10100 Balikesir, Turkey

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2012年 / 40卷 / 06期

关键词：

Galleria mellonella; prophenol oxidase; affinity chromatography; inhibition; PRO-PHENOL OXIDASE; POLYPHENOL OXIDASE; SUBSTRATE-SPECIFICITY; MANDUCA-SEXTA; PHENOLOXIDASE ACTIVITY; HELIOTHIS-VIRESCENS; HEAT INACTIVATION; ACTIVATING SYSTEM; DIPHENOL OXIDASE; INHIBITION;

D O I：

10.3109/10731199.2012.696060

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Prophenoloxidase (PPO) was purified from Galleria mellonella L. A 67-fold purification of the proenzyme with 352% yield was achieved by using a Sepharose 4B-L-tyrosine-p-amino benzoic acid affinity column. The purified enzyme was migrated as a single band on SDS-polyacrylamide gel electrophoresis. Km and V-max values were 0.017 M and 1430.45 EU for catechol. Inhibition of PPO was investigated with inhibitors such as p-aminobenzoic acid, etyleneglycol, and ascorbic acid. Among them, ascorbic acid showed the strongest inhibitory activity with IC50 value of 2.94 mu M. The current paper represents new strategies for the biological control of the Galleria mellonella L. insect.

引用

页码：391 / 395

页数：5

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