Associations of DNMT3B-149C>T and-2437T>A polymorphisms and lung cancer risk in Chinese population

Background: DNMT3B polymorphisms are associated with the susceptibility of lung cancer. DNMT3B - 2437T>A is a novel polymorphism, and its influence on the risk of lung cancer in Chinese was investigated in this study. In addition, effect of DNMT3B - 149C>T polymorphism on lung cancer was also explored. Methods: Genotyping in subjects were performed by PCR-RFLP. Haplotype frequencies were estimated by estimating haplotype software. Adjusted odds ratios ( ORs) with 95% confidence intervals ( CIs) were calculated by unconditional logistic regression analysis. Results: Neither of the two polymorphisms was correlated with lung cancer (-149C>T: CT+TT vs CC: OR = 0.78, 95% CI, 0.57 to 1.05, P = 0.361; - 2437T>A: AT+AA vs TT: OR = 0.99, 95% CI, 0.74 to 1.33, P = 0.168). In stratification analysis, T-allele carrier genotype of -149C>T polymorphism resulted in a reduced lung cancer risk at stage II, compared with CC ( OR = 0.46, 95% CI, 0.28 to 0.77, P = 0.023). In haplotype analysis, when -149C/-2437T was used as reference, the other combined genotypes of the two polymorphisms had no significant effect on lung cancer risk ( P > 0.05). Conclusions: The two DNMT3B polymorphisms are not correlated with lung cancer risk among Chinese population nor the haplotype of them.