Line-Broadening in Low-Temperature Solid-State NMR Spectra of Fibrils

被引：26

作者：

Bauer, Thomas ^{[1
]}

Dotta, Claudio ^{[1
]}

Balacescu, Livia ^{[1
]}

Gath, Julia ^{[1
]}

Hunkeler, Andreas ^{[1
]}

Bockmann, Anja ^{[2
]}

Meier, Beat H. ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, Phys Chem, Vladimir Prelog Weg 2, CH-8093 Zurich, Switzerland

[2] Univ Lyon 1, CNRS, UMR 5086, Inst Biol & Chim Prot, 7 Passage Vercors, F-69367 Lyon, France

来源：

JOURNAL OF BIOMOLECULAR NMR | 2017年 / 67卷 / 01期

基金：

瑞士国家科学基金会;

关键词：

Solid-state NMR; Low-temperature; Line broadening; Fibrils; Protein; HET-s; DYNAMIC NUCLEAR-POLARIZATION; HET-S PRION; HET-S(218-289) AMYLOID FIBRILS; ATOMIC-RESOLUTION STRUCTURE; ANTIFREEZE PROTEIN; PODOSPORA-ANSERINA; MAS NMR; SPECTROSCOPY; SCHEME; ICE;

D O I：

10.1007/s10858-016-0083-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The temperature-dependent resonance-line broadening of HET-s(218-289) in its amyloid form is investigated in the range between 110 K and 280 K. Significant differences are observed between residues in the structured hydrophobic triangular core, which are broadened the least and can be detected down to 100 K, and in the solvent-exposed parts, which are broadened the most and often disappear from the observed spectrum around 200 K. Below the freezing of the bulk water, around 273 K, the protein fibrils are still surrounded by a layer of mobile water whose thickness decreases with temperature, leading to drying out of the fibrils.

引用

页码：51 / 61

页数：11

共 50 条

[21] Solid-State NMR Techniques for the Structural Determination of Amyloid Fibrils
Chan, Jerry C. C.
SOLID STATE NMR, 2012, 306 : 47 - 88
[22] Structural Characterization of Polyglutamine Fibrils by Solid-State NMR Spectroscopy
Schneider, Robert
Schumacher, Miria C.
Mueller, Henrik
Nand, Deepak
Klaukien, Volker
Heise, Henrike
Riedel, Dietmar
Wolf, Gerhard
Behrmann, Elmar
Raunser, Stefan
Seidel, Ralf
Engelhard, Martin
Baldus, Marc
JOURNAL OF MOLECULAR BIOLOGY, 2011, 412 (01) : 121 - 136
[23] Emerging Structural Understanding of Amyloid Fibrils by Solid-State NMR
Meier, Beat H.
Riek, Roland
Bockmann, Anja
TRENDS IN BIOCHEMICAL SCIENCES, 2017, 42 (10) : 777 - 787
[24] LOW-TEMPERATURE DEACTIVATION OF SINGLET OXYGEN ON SOLID-STATE SURFACES
RYSKIN, ME
CHERNYSH, VI
KURENEVA, TY
KHIMICHESKAYA FIZIKA, 1990, 9 (02): : 163 - 173
[25] POLYSELENIDE COMPLEXES AS LOW-TEMPERATURE PRECURSORS TO SOLID-STATE CHALCOGENIDES
KIM, KW
DHINGRA, S
KANATZIDIS, MG
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1990, 199 : 141 - INOR
[26] A LOW-TEMPERATURE SOLID-STATE PHOTO-ACOUSTIC SPECTROMETER
IRONSIDE, CN
DENNING, RG
JOURNAL OF PHYSICS E-SCIENTIFIC INSTRUMENTS, 1982, 15 (01): : 142 - 144
[27] LOW-POWER MULTIPULSE LINE NARROWING IN SOLID-STATE NMR
BURUM, DP
LINDER, M
ERNST, RR
JOURNAL OF MAGNETIC RESONANCE, 1981, 44 (01) : 173 - 188
[28] LINE-BROADENING EFFECTS FOR XE-129 ABSORBED IN THE AMORPHOUS STATE OF SOLID POLYMERS
KENTGENS, APM
VANBOXTEL, HA
VERWEEL, RJ
VEEMAN, WS
MACROMOLECULES, 1991, 24 (12) : 3712 - 3714
[29] HIGH-RESOLUTION LOW-TEMPERATURE SOLID-STATE NMR-STUDIES OF CARBONIUM-IONS
YANNONI, CS
MYHRE, PC
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1991, 201 : 8 - PHYS
[30] Anion rotation and cation diffusion in low-temperature sodium orthophosphate: Results from solid-state NMR
Witschas, M
Eckert, H
Freiheit, H
Putnis, A
Korus, G
Jansen, M
JOURNAL OF PHYSICAL CHEMISTRY A, 2001, 105 (28): : 6808 - 6816

← 1 2 3 4 5 →