Do Surface Defects and Modification Determine the Observed Toxicity of Carbon Nanotubes?

被引：15

作者：

Senanayake, V. ^{[2
]}

Juurlink, B. H. ^{[2
]}

Zhang, C. ^{[3
]}

Zhan, E. ^{[3
]}

Wilson, L. D. ^{[4
]}

Kwon, J. ^{[4
]}

Yang, J. ^{[5
]}

Lim, Z. L. ^{[1
]}

Brunet, S. M. K. ^{[6
]}

Schatte, G. ^{[6
]}

Maley, J. M. ^{[6
]}

Hoffmeyer, R. E. ^{[7
]}

Sammynaiken, R. ^{[1
,6
]}

机构：

[1] Univ Saskatchewan, Dept Biochem, Saskatoon, SK S7N 5E5, Canada

[2] Univ Saskatchewan, Dept Anat & Cell Biol, Saskatoon, SK S7N 5E5, Canada

[3] Univ Saskatchewan, Dept Mech Engn, Saskatoon, SK S7N 5A9, Canada

[4] Univ Saskatchewan, Dept Chem, Saskatoon, SK S7N 5C9, Canada

[5] Univ Saskatchewan, Coll Pharm & Nutr, Saskatoon, SK S7N 5C9, Canada

[6] Univ Saskatchewan, Saskatchewan Struct Sci Ctr, Saskatoon, SK S7N 5C9, Canada

[7] Quantum Solut, Saskatoon, SK S7H 3Y7, Canada

来源：

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2008年 / 4卷 / 04期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Two-Photon Laser Scanning Confocal Microscopy; Raman Microscopy; Toxicology; LDH Assay; C6; Cells; AM11; Carbon Nanotubes; Asbestos; Surface Defect; Surface Charge;

D O I：

10.1166/jbn.2008.017

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The role of charge and surface modification in the toxicity of CNT is investigated. Two-photon confocal microscopy, Raman microscopy and Lactate Dehydrogenase (LDH) assay are employed to study the effect of CNT on Rat glioma cell line (C6 cells) and rat alveolar macrophage continuous cell line (AM 11). Two-photon confocal microscopy reveals that unmodified CNT is toxic to the C6 cell line but when modified by DNA it becomes relatively non-toxic. Both LDH assay and Raman microscopy show that CNT dispersed in serum is not toxic to the AM11 and C6 cell lines. We have illustrated that the controversy in CNT toxicity will continue, even within the same laboratory, if the surface properties of nanomaterials both before and after modification are not considered and understood before these materials are tested for toxicological properties.

引用

页码：515 / 523

页数：9

共 54 条

[1] Applications of Carbon Nanotubes in Biotechnology and Biomedicine [J].

Bekyarova, Elena ;

Ni, Yingchun ;

Malarkey, Erik B. ;

Montana, Vedrana ;

McWilliams, Jared L. ;

Haddon, Robert C. ;

Parpura, Vladimir .

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2005, 1 (01) :3-17

[2] Mitochondrial changes induced by natural and synthetic asbestos fibers: Studies on isolated mitochondria [J].

Bergamini, C ;

Fato, R ;

Biagini, G ;

Pugnaloni, A ;

Glantomassi, F ;

Foresti, E ;

Lesci, GI ;

Roveri, N ;

Lenaz, G .

CELLULAR AND MOLECULAR BIOLOGY, 2004, 50 :691-700

[3] Applications of carbon nanotubes in drug delivery [J].

Bianco, A ;

Kostarelos, K ;

Prato, M .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2005, 9 (06) :674-679

[4] Probing the interaction of single walled carbon nanotubes within cell culture medium as a precursor to toxicity testing [J].

Casey, A. ;

Davoren, M. ;

Herzog, E. ;

Lyng, F. M. ;

Byrne, H. J. ;

Chambers, G. .

CARBON, 2007, 45 (01) :34-40

[5] Detection of 8-oxo-2'-deoxyguanosine, a marker of oxidative DNA damage, in culture medium from human mesothelial cells exposed to crocidolite asbestos [J].

Chen, Q ;

Marsh, J ;

Ames, B ;

Mossman, B .

CARCINOGENESIS, 1996, 17 (11) :2525-2527

[6] Luminescence enhancement of ZnS:Mn nanoclusters in zeolite [J].

Chen, W ;

Sammynaiken, R ;

Huang, YN .

JOURNAL OF APPLIED PHYSICS, 2000, 88 (09) :5188-5193

[7] Using nanoparticles to enable simultaneous radiation and photodynamic therapies for cancer treatment [J].

Chen, W ;

Zhang, J .

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2006, 6 (04) :1159-1166

[8] Photoluminescence and photostimulated luminescence of Tb3+ and Eu3+ in zeolite-Y [J].

Chen, W ;

Sammynaiken, R ;

Huang, Y .

JOURNAL OF APPLIED PHYSICS, 2000, 88 (03) :1424-1431

[9] Nanoparticle fluorescence based technology for biological applications [J].

Chen, Wei .

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2008, 8 (03) :1019-1051

[10] Asbestos fibers and interleukin-1 upregulate the formation of reactive nitrogen species in rat pleural mesothelial cells [J].

Choe, N ;

Tanaka, S ;

Kagan, E .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1998, 19 (02) :226-236

← 1 2 3 4 5 6 →