Silver-dendrimer nanocomposites as oligonucleotide labels for electrochemical stripping detection of DNA hybridization

被引：30
作者：
Jin, Xin [1 ]
Zhou, Ling [1 ]
Zhu, Bo [1 ]
Jiang, Xue [1 ]
Zhu, Ningning [1 ]
机构：
[1] Shanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Guilin Rd 100, Shanghai 200234, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2018年 / 107卷
关键词：
Electrochemical; Labels; DNA hybridization; Silver-dendrimer nanocomposites; HEPATITIS-B-VIRUS; ELECTRODES; BIOSENSOR; INDICATOR; AMPLIFICATION; NANOPARTICLES; STRATEGY; BLUE;
D O I：
10.1016/j.bios.2018.02.033
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Silver-dendrimer nanocomposites were synthesized and used as oligonucleotide labels for electrochemical stripping detection of DNA hybridization. The synthesized silver-dendrimer nanocomposites were characterized by UV-vis spectrophotometry, X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). Ratios of silver/dendrimer were optimized in order to obtain stable nanocomposites with maximal silver loading in the interior of a polymeric shell. The silver-dendrimer nanocomposites were attached to sequence-known DNA probes specific to colitoxin, and used to detect probe hybridization by dissolution of the silver nanoparticles in the interior of dendrimer in a diluted nitric acid, followed by measurement of Ag+ ions by anodic stripping voltammetry (ASV). Use of differential pulse voltammetry for the stripping step, along with optimization of the ASV conditions, enabled a detection limit of 0.78 pM. The present strategy, in combination with dendrimer-encapsulated copper labeled oligonucleotides probe reported previously, could potentially be used to detect single or multiple DNA targets in one sample.
引用
收藏
页码：237 / 243
页数：7
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