Diuretic activity of some fractions of the epidermis of Poria cocos

被引：100

作者：

Feng, Ya-Long ^{[1
]}

Lei, Ping ^{[4
]}

Tian, Ting ^{[1
]}

Yin, Lu ^{[1
]}

Chen, Dan-Qian ^{[1
]}

Chen, Hua ^{[1
]}

Mei, Qibing ^{[2
]}

Zhao, Ying-Yong ^{[1
,2
]}

Lin, Rui-Chao ^{[3
]}

机构：

[1] Northwest Univ, Coll Life Sci, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Shaanxi, Peoples R China

[2] Fourth Mil Med Univ, Sch Pharm, Dept Pharmacol, Xian 710032, Shaanxi, Peoples R China

[3] State Food & Drug Adm, Natl Inst Food & Drug Control, Beijing 100050, Peoples R China

[4] Shaanxi Microbiol Res Inst, Xian 710043, Shaanxi, Peoples R China

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2013年 / 150卷 / 03期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Poria cocos; Bioactive fractions; Diuretic activity; Urinary excretion; Electrolyte excretion; SURFACE-LAYER; RENAL INJURY; TRITERPENES; ETHANOL; EXTRACT; HOELEN; CELLS; RATS;

D O I：

10.1016/j.jep.2013.10.043

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ethnopharmacological relevance: Poria cocos Wolf (Polyporaceae) is a well-known medicinal fungus, the epidermis ("Fu-Ling-Pi" in Chinese) of the sclerotia is used as a diuretic for treating oedema and promoting the diuretic process. In this paper we report on the diuretic activity in rats of petroleum ether, ethyl acetate, n-butanol and the remaining fractions of the ethanol extract from the epidermis of Poria cocos. Materials and methods: Petroleum ether, ethyl acetate, n-butanol and the remaining fractions of the ethanol extract of Fu-Ling-Pi were orally administered to rats. The urinary excretion rate and the pH and electrolyte excretion were measured in the urine of saline-loaded rats. Results: In this study, all the tested fractions of Fu-Ling-Pi increased the urinary excretion rate. The three doses of the ethyl acetate fraction all produced remarkable urinary output in 6 h, and all produced a remarkable increase in Na+ excretion and Cl- excretion. The Na+/K+ value in the experimental group was significantly enhanced compared with that of the control group, but the three doses of the ethyl acetate fraction had no effect on the K+ excretion. The 25-mg/kg and 50-mg/kg doses of the n-butanol fraction showed notable urinary output and produced a remarkable increase of Na+ excretion and Cl- excretion, but the two doses did not produce a remarkable effect on the Na+/K+ value. The petroleum ether and remaining fractions did not show remarkable diuretic activities compared with the control group. Conclusions: This study confirmed that the ethyl acetate and n-butanol fractions present a remarkable diuretic effect, showing that they are the diuretic bioactive fractions of Fu-Ling-Pi. This finding appears to indicate at least two mechanisms for the observed diuretic activity, and the K+-saving diuretic effect may be related to the triterpenoid components of intermediate polarity contained in this fungus, particularly the lanostanes tetracyclic triterpenoids. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

引用

页码：1114 / 1118

页数：5

共 28 条

[1] Triterpene acids from Poria cocos and their anti-tumor-promoting effects [J].