共 21 条
- [21] Hydrocarbon stapled tryptophan and arginine-rich antimicrobial peptides may act through a membrane-disruptive mechanismABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2015, 250Jenner, ZacharyGonzalez, MartinBruns, Kerry
Correlations between membrane immersion depth, orientation, and salt-resistance of tryptophan-rich antimicrobial peptides
被引：19
|作者：
Yu, Hui-Yuan
[1
,2
]
Yip, Bak-Sau
[1
,2
,3
]
Tu, Chih-Hsiang
[1
,2
]
Chen, Heng-Li
[1
,2
]
Chu, Hung-Lun
[1
,2
]
Chih, Ya-Han
[1
,2
]
Cheng, Hsi-Tsung
[1
,2
]
Sue, Shih-Che
[4
,5
]
Cheng, Jya-Wei
[1
,2
]
机构：
[1] Natl Tsing Hua Univ, Inst Biotechnol, Hsinchu 300, Taiwan
[2] Natl Tsing Hua Univ, Dept Med Sci, Hsinchu 300, Taiwan
[3] Natl Taiwan Univ Hosp, Hsinchu Branch, Dept Neurol, Hsinchu 300, Taiwan
[4] Natl Tsing Hua Univ, Inst Bioinformat & Struct Biol, Hsinchu 300, Taiwan
[5] Natl Tsing Hua Univ, Dept Life Sci, Hsinchu 300, Taiwan
来源：
关键词：
Antimicrobial peptide;
Tryptophan-rich;
Salt-resistance;
NMR;
PRE;
MICELLE-BOUND PEPTIDES;
PSEUDOMONAS-AERUGINOSA;
RATIONAL DESIGN;
CYSTIC-FIBROSIS;
HYDROPHOBICITY;
ANTIBACTERIAL;
MECHANISM;
CHARGE;
NMR;
PERMEABILIZATION;
D O I：
10.1016/j.bbamem.2013.07.020
中图分类号：
Q5 [生物化学];
Q7 [分子生物学];
学科分类号：
071010 ;
081704 ;
摘要：
The efficacies of many antimicrobial peptides are greatly reduced in the presence of high salt concentrations therefore limiting their development as pharmaceutical compounds. PEM-2-W5K/A9W, a short Trp-rich antimicrobial peptide developed based on the structural studies of PEM-2, has been shown to be highly active against various bacterial strains with less hemolytic activity. Here, correlations between membrane immersion depth, orientation, and salt-resistance of PEM-2 and PEM-2-W5K/A9W have been investigated via solution structure and paramagnetic resonance enhancement studies. The antimicrobial activities of PEM-2-W5K/A9W and PEM-2 against various bacterial and fungal strains including multidrug-resistant and clinical isolates under high salt conditions were tested. The activities of the salt-sensitive peptide PEM-2 were reduced and diminished at high salt concentrations, whereas the activities of PEM-2-W5K/A9W were less affected. The results indicated that the strong salt-resistance of PEM-2-W5K/A9W may arise from the peptide positioning itself deeply into microbial cell membranes and thus able to disrupt the membranes more efficiently. (C) 2013 Elsevier B.V. All rights reserved.
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页码：2720 / 2728
页数：9
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