Taurochenodeoxycholic Acid Suppresses NF-κB Activation and Related Cytokines Expression in Peritoneal Macrophages from Adjuvant Arthritis Rat

Taurochenodeoxycholic acid (TCDCA) is one of main bioactive substances of animals' bile acid and confers good anti-inflammatory activity. This study aimed to investigate the mechanism of the anti-adjuvant arthritis activity of TCDCA, the actions were observed in vitro on the protein and mRNA expression of cytokines, the activity of nuclear factor-kappa B (NF-kappa B) and its inhibitory I kappa B alpha protein level of peritoneal macrophages (PMs) in adjuvant arthritis (AA) rats. In a definite concentration ranging from 150 mu g/mL to 200 mu g/mL, the over protein and mRNA expression of TNF-alpha, IL-1 beta and IL-6 were remarkably suppressed in the supernatant of PMs treatment with TCDCA. Positive correlations were found between changes of NF-kappa B activity and expression of TNF-alpha, IL-1 beta and IL-6 influenced by TCDCA. The activity of NF-kappa B was markedly inhibited and I kappa B alpha protein level was increased by TCDCA (150 mu g/mL, 180 mu g/mL and 200 mu g/mL). TCDCA suppresses the protein and mRNA expression of TNF-alpha, IL-1 beta, and IL-6 by inhibiting the NF-kappa B binding activity, which is mediated through up-regulating the I kappa B alpha expression of PMs in AA rats.