Development of a Sensitive Monoclonal Antibody-Based Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Determination of Monensin in Edible Chicken Tissues

被引：9

作者：

Li, Langhong ^{[1
,2
]}

Pan, Yuanhu ^{[1
,2
]}

Tao, Yanfei ^{[1
,2
]}

Chen, Donmei ^{[1
,2
]}

Wang, Yulian ^{[1
,2
]}

Wang, Xu ^{[1
,2
]}

Liu, Zhenli ^{[1
,2
]}

Peng, Dapeng ^{[1
,2
]}

Yuan, Zonghui ^{[1
,2
]}

机构：

[1] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues HZAU, Wuhan 430070, Hubei, Peoples R China

[2] Huazhong Agr Univ, MOA Key Lab Detect Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China

来源：

FOOD ANALYTICAL METHODS | 2019年 / 12卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Monensin; Monoclonal antibody; Indirect competitive enzyme-linked immunosorbent assay; Edible chicken tissues; RESIDUES; COCCIDIOSTATS; CHROMATOGRAPHY; VALIDATION; ELISA; EGGS; FOOD;

D O I：

10.1007/s12161-019-01461-3

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

To monitor monensin (MON) residues in chicken tissues, a monoclonal antibody (mAb)-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed in this study. The haptenMON was obtained by adding hydrochloric acid to a MON salt, and then was coupled with carrier protein. After the inoculation of female Balb/c mice and cell fusions, one cell line, MON/4C9, was obtained. The MON/4C9 antibody exhibited the ability to specifically recognize MON with IC50 1.28 mu g L-1.Based on this mAb, an optimized ic-ELISA protocol was performed using only methanol-water (8:2, v/v) in chicken tissue samples. The limits of detection and the limits of quantification of MON in various sample matrices varied from 0.38 to 1.01 mu g kg(-1). The recoveries ranged from 71.9 to 116.9% in chicken tissues, and the intra- and inter-assay CVs were all less than 18%. Moreover, the developed method also exhibited a positive correlation with the results of HPLC-MS conducted on the samples. These results suggest that the prepared mAb and the developed ic-ELISA method will be a useful tool for detecting MON in chicken tissues.

引用

页码：1479 / 1486

页数：8

共 21 条

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