A Molecular Beacon-Based Signal-Off Surface-Enhanced Raman Scattering Strategy for Highly Sensitive, Reproducible, and Multiplexed DNA Detection

被引：84
作者：
Wei, Xinpan [1 ,2 ]
Su, Shao [1 ,2 ,3 ]
Guo, Yuanyuan [1 ,2 ]
Jiang, Xiangxu [1 ,2 ]
Zhong, Yiling [1 ,2 ]
Su, Yuanyuan [1 ,2 ]
Fan, Chunhai [3 ]
Lee, Shuit-Tong [1 ,2 ]
He, Yao [1 ,2 ]
机构：
[1] Soochow Univ, Inst Funct Nano & Soft Mat FUNSOM, Suzhou 215123, Peoples R China
[2] Soochow Univ, Jiangsu Key Lab Carbon Based Funct Mat & Devices, Suzhou 215123, Peoples R China
[3] Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China
来源：
SMALL | 2013年 / 9卷 / 15期
基金：
中国博士后科学基金;
关键词：
DNA; molecular beacons; nanowires; oligonucleotides; surface-enhanced Raman scattering; SILICON NANOWIRES; NANOPARTICLES; SPECTROSCOPY; SINGLE; SENSOR; SUBSTRATE; PROTEINS; PLATFORM; DIMERS; RANGE;
D O I：
10.1002/smll.201202914
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Stem-loop oligonucleotides tagged with organic dyes are employed as capture and reporter probes in a molecular beacon-based signal-off surface- enhanced Raman scattering (SERS) strategy. DNA strands are immobilized on a gold nanoparticle-decorated silicon nanowire array (AuNPs@SiNWAr). DNA with a concentration down to ≈10 fM is detected under irradiation from a low-power laser, which is better than or comparable to that detected by signal-on SERS methods. The signal-off strategy allows identification of single-base mismatches and detection of multiplexed DNA. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：2493 / 2499
页数：7
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