Isolation, identification of sludge-lysing strain and its utilization in thermophilic aerobic digestion for waste activated sludge

被引：81

作者：

Li, Xuesong ^{[1
]}

Ma, Hongzhi ^{[2
]}

Wang, Qunhui ^{[2
]}

Matsumoto, Shoichiro ^{[1
]}

Maeda, Toshinari ^{[1
]}

Ogawa, Hiroaki I. ^{[1
]}

机构：

[1] Kyushu Inst Technol, Grad Sch Life Sci & Syst Engn, Dept Biol Funct & Engn, Wakamatsu Ku, Kitakyushu, Fukuoka 8080196, Japan

[2] Univ Sci & Technol Beijing, Dept Environm Engn, Beijing 100083, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 09期

基金：

日本学术振兴会;

关键词：

Thermophilic aerobic digestion; Thermophilic bacteria; Protease; Activated waste sludge; POLYMERIC SUBSTANCES EPS; ANAEROBIC-DIGESTION; SEWAGE-SLUDGE; ULTRASONIC PRETREATMENT; BACTERIA;

D O I：

10.1016/j.biortech.2008.12.019

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A strain of sludge-lysing bacteria was isolated from waste activated sludge (WAS) in this study. The result of 16S rRNA gene analysis demonstrated that it was a species of new genus Brevibacillus (named Brevibacillus sp. KH3). The strain could release the protease with molecule weight of about 40 kDa which could enhance the efficiency of sludge thermophilic aerobic digestion. During the sterilized sludge digestion experiment inoculated with Brevibacillus sp. KH3, the maximum protease activity was 0.41 U/ml at pH 8 and 50 C, and Maximum TSS removal ratio achieved 32.8% after 120 h digestion at pH 8 and 50 C. In the case of un-sterilized sludge digestion inoculated with Brevibacillus sp. KH3, TSS removal ratio in inoculated-group was 54.8%, increasing at 11.86% compared with un-inoculation (46.2%). The result demonstrated that inoculation of Brevibacillus sp. KH3 could help to degrade the EPS and promote the collapse of cells and inhibit the growth of certain kinds of microorganisms. It indicated that Brevibacillus sp. KH3 strain had a high potential to enhance WAS-degradation efficiency in thermophilic aerobic digestion. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2475 / 2481

页数：7

共 31 条

[1]

APHA/AWWA/WEF, 2017, Standard Methods for the Examination of Water and Wastewater, V23rd

[2] Principles and potential of the anaerobic digestion of waste-activated sludge [J].