Fabrication of Arrayed Flexible Screen-Printed Glucose Biosensor Based on Microfluidic Framework

This paper uses a simple, fast, and low-cost method to fabricate an arrayed flexible glucose biosensor device. The ruthenium dioxide (RuO2) sensing membranes were deposited on the flexible polyethylene terephthalate substrate by radio frequency sputtering technology. The silver paste was fabricated as conducting wire and the epoxy was fabricated as insulation layer by screen-printed technique. The glucose oxidase and nafion were prepared as glucose sensing membrane. The glucose sensing membranes were dropped on RuO2 sensing membranes by entrapment method. The arrayed flexible glucose biosensor was measured in different concentrations of glucose solution. Finally, the arrayed flexible glucose biosensor was integrated in microfluidic to measure characteristics at the dynamic condition. All experimental results demonstrated that the arrayed flexible glucose biosensor based on microfluidic had a fast potentiometric response (<10 s) to glucose, high sensitivity, and a good linear voltage-time relation over a wide range of glucose concentrations from 100 to 400 mg/dL.