A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000

被引：112

作者：

Almeida, Nalvo F. ^{[2
]}

Yan, Shuangchun ^{[1
]}

Lindeberg, Magdalen ^{[3
]}

Studholme, David J. ^{[4
]}

Schneider, David J. ^{[5
]}

Condon, Bradford ^{[3
]}

Liu, Haijie ^{[1
]}

Viana, Carlos J.

Warren, Andrew ^{[2
]}

Evans, Clive ^{[2
]}

Kemen, Eric ^{[4
]}

MacLean, Dan ^{[4
]}

Angot, Aurelie ^{[3
,6
]}

Martin, Gregory B. ^{[3
,6
]}

Jones, Jonathan D. ^{[4
]}

Collmer, Alan ^{[3
]}

Setubal, Joao C. ^{[2
,7
]}

Vinatzer, Boris A. ^{[1
]}

机构：

[1] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA

[2] Virginia Tech, Virginia Bioinformat Inst, Blacksburg, VA 24061 USA

[3] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA

[4] Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

[5] USDA ARS, Ithaca, NY 14853 USA

[6] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA

[7] Virginia Tech, Dept Comp Sci, Blacksburg, VA 24061 USA

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2009年 / 22卷 / 01期

基金：

美国国家科学基金会;

关键词：

effector-triggered immunity; nonhost resistance; pyrosequencing; Solexa sequencing; DISEASE RESISTANCE GENE; SECRETION SYSTEM; INNATE IMMUNITY; ARABIDOPSIS PLANTS; PHASEOLICOLA; 1448A; NONHOST RESISTANCE; NATURAL VARIATION; BASAL DEFENSE; IDENTIFICATION; VIRULENCE;

D O I：

10.1094/MPMI-22-1-0052

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Diverse gene products including phytotoxins, pathogen-associated molecular patterns, and type III secreted effectors influence interactions between Pseudomonas syringae strains and plants, with additional yet uncharacterized factors likely contributing as well. Of particular interest are those interactions governing pathogen-host specificity. Comparative genomics of closely related pathogens with different host specificity represents an excellent approach for identification of genes contributing to host-range determination. A draft genome sequence of Pseudomonas syringae pv. tomato T1, which is pathogenic on tomato but nonpathogenic on Arabidopsis thaliana, was obtained for this purpose and compared with the genome of the closely related A. thaliana and tomato model pathogen P. syringae pv. tomato DC3000. Although the overall genetic content of each of the two genomes appears to be highly similar, the repertoire of effectors was found to diverge significantly. Several P. syringae pv. tomato T1 effectors absent from strain DC3000 were confirmed to be translocated into plants, with the well-studied effector AvrRpt2 representing a likely candidate for host-range determination. However, the presence of avrRpt2 was not found sufficient to explain A. thaliana resistance to P. syringae pv. tomato T1, suggesting that other effectors and possibly type III secretion system-independent factors also play a role in this interaction.

引用

页码：52 / 62

页数：11

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