Assessment of first-rate adsorption constants of platelet membrane proteins bearing liposomes by surface tension measurements

The presence of platelet proteins embedded in liposome membranes drastically modified the diffusional transport and adsorption of these vesicles at the air/water interface. The calculated first-order rate constants of adsorption were by a factor of 10 higher than those corresponding to protein-free liposomes. These results provide an indication of the possible use of such proteoliposomes as models to obtain a more fundamental understanding at the molecular level of the interaction of proteins with phospholipids during adsorption and spreading processes. (C) 1999 Elsevier Science B.V. All rights reserved.