PET Imaging of Stroke-Induced Neuroinflammation in Mice Using [18F]PBR06

被引：42

作者：

Lartey, Frederick M. ^{[1
]}

Ahn, G-One ^{[1
,6
]}

Shen, Bin ^{[3
]}

Cord, Keith-Travis ^{[1
]}

Smith, Tenille ^{[4
,10
,11
]}

Chua, Joshua Y. ^{[4
]}

Rosenblum, Sahar ^{[4
,10
,11
]}

Liu, Hongguang ^{[3
]}

James, Michelle L. ^{[3
]}

Chernikova, Sophia ^{[1
]}

Lee, Star W. ^{[1
,4
,9
]}

Pisani, Laura J. ^{[3
]}

Tirouvanziam, Rabindra ^{[5
,12
]}

Chen, John W. ^{[7
,8
]}

Palmer, Theo D. ^{[13
]}

Chin, Frederick T. ^{[3
]}

Guzman, Raphael ^{[4
,10
,11
]}

Graves, Edward E. ^{[1
,2
]}

Loo, Billy W., Jr. ^{[1
,2
]}

机构：

[1] Stanford Univ, Dept Radiat Oncol, Sch Med, Stanford, CA 94305 USA

[2] Stanford Canc Inst, Stanford, CA USA

[3] Stanford Univ, Dept Radiol, Sch Med, Stanford, CA 94305 USA

[4] Stanford Univ, Dept Neurosurg, Sch Med, Stanford, CA 94305 USA

[5] Stanford Univ, Dept Pediat, Sch Med, Stanford, CA 94305 USA

[6] Pohang Univ Sci & Technol, Kyungbuk 790784, South Korea

[7] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Ctr Syst Biol, Boston, MA USA

[8] Harvard Univ, Sch Med, Dept Radiol, Massachusetts Gen Hosp, Boston, MA 02115 USA

[9] Salk Inst Biol Studies, Genet Lab, La Jolla, CA 92037 USA

[10] Univ Basel, Dept Neurosurg, Basel, Switzerland

[11] Univ Basel, Dept Biomed, Basel, Switzerland

[12] Emory Univ, Sch Med, Dept Pediat, Emory Childrens Ctr CF Res, Atlanta, GA USA

[13] Stanford Univ, Inst Stem Cell Biol & Regenerat Med, Sch Med, Stanford, CA 94305 USA

来源：

MOLECULAR IMAGING AND BIOLOGY | 2014年 / 16卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Stroke; Neuroinflammation; Translocator protein 18 kDa (TSPO); Peripheral benzodiazepine receptor (PBR); Positron emission tomography (PET); F-18]PBR06; PROTEIN; 18; KDA; PERIPHERAL BENZODIAZEPINE-RECEPTORS; POSITRON-EMISSION-TOMOGRAPHY; IN-VIVO; INFLAMMATORY RESPONSE; CEREBRAL-ISCHEMIA; BRAIN; MICROGLIA; LIGAND; PERMANENT;

D O I：

10.1007/s11307-013-0664-5

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

The purpose of this study is to evaluate the 18 kDa translocator protein (TSPO) radioligand [F-18]N-fluoroacetyl-N-(2,5-dimethoxybenzyl)-2-phenoxyaniline ([F-18]PBR06) as a positron emission tomography (PET) imaging biomarker of stroke-induced neuroinflammation in a rodent model. Stroke was induced by transient middle cerebral artery occlusion in Balb/c mice. Dynamic PET/CT imaging with displacement and preblocking using PK111195 was performed 3 days later. PET data were correlated with immunohistochemistry (IHC) for the activated microglial markers TSPO and CD68 and with autoradiography. [F-18]PBR06 accumulation peaked within the first 5 min postinjection, then decreased gradually, remaining significantly higher in infarct compared to noninfarct regions. Displacement or preblocking with PK11195 eliminated the difference in [F-18]PBR06 uptake between infarct and noninfarct regions. Autoradiography and IHC correlated well spatially with uptake on PET. [F-18]PBR06 PET specifically images TSPO in microglial neuroinflammation in a mouse model of stroke and shows promise for imaging and monitoring microglial activation/neuroinflammation in other disease models.

引用

页码：109 / 117

页数：9

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