Invivo chondrocyte and transforming growth factor-1 delivery using the thermosensitive chitosan/starch/-glycerol phosphate hydrogel

被引：12
|
作者：
Faikrua, Atchariya [1 ]
Wittaya-areekul, Sakchai [1 ]
Oonkhanond, Bovornlak [2 ]
Viyoch, Jarupa [1 ]
机构：
[1] Naresuan Univ, Dept Pharmaceut Technol, Phitsanulok 65000, Thailand
[2] Mahidol Univ, Dept Chem Engn, Nakhon Pathom, Thailand
来源：
JOURNAL OF BIOMATERIALS APPLICATIONS | 2013年 / 28卷 / 02期
关键词：
Chitosan; starch; -glycerol phosphate; thermosensitive hydrogel; transforming growth factor-1; chondrocytes; CARTILAGE TISSUE; ARTICULAR-CARTILAGE; GROWTH-FACTOR-BETA-1; MICROSPHERES; EXPRESSION; MATRICES; PHENOTYPE; SCAFFOLD; RELEASE;
D O I：
10.1177/0885328212441847
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
In present study, the chitosan/starch/-glycerol phosphate hydrogel was investigated as an effective carrier for chondrocytes and delivery of transforming growth factor-1. Invitro study indicated that transforming growth factor-1 was released sustainably for 14 days with its biological activity to stimulate chondrocyte functions, as indicated by the strong expression of type II collagen protein. Subcutaneous implantation to rats revealed the strong expressions of type II collagen and aggrecan messenger ribonucleic acids, and also type II collagen protein was observed in the hydrogel in combination with transforming growth factor-1 within 2 weeks. Our collective results showed the potential of chitosan/starch/-glycerol phosphate hydrogel for effective delivery of chondrocytes and transforming growth factor-1, and preserve chondrocytes' phenotype and functions invitro.
引用
收藏
页码：175 / 186
页数：12
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