Laboratory methods to study African swine fever virus

被引：53
|
作者：
de Leon, Patricia [1 ]
Bustos, Maria J. [1 ]
Carrascosa, Angel L. [1 ]
机构：
[1] Univ Autonoma Madrid, CSIC, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain
来源：
VIRUS RESEARCH | 2013年 / 173卷 / 01期
关键词：
African swine fever virus (ASFV); Production; Susceptibility; Target cells; Purification; Titration; Deletion mutants; Swine macrophages; WSL; ASF vaccine; CELL-LINES; VIRULENCE DETERMINANT; EFFICIENT REPLICATION; ALVEOLAR MACROPHAGES; GENETIC MANIPULATION; NUCLEAR-FACTOR; DOMESTIC PIGS; HEMADSORPTION; EXPRESSION; ACTIVATION;
D O I：
10.1016/j.virusres.2012.09.013
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We summarize findings of comparative studies in different cells cultures susceptible to ASFV infection, through the analysis of virus components and infectious virus particles production, as alternative means to grow field and laboratory ASFV strains. We also provide different methods to assay the infectivity of ASFV samples and to purify the infective virus particles. Finally we describe the general strategy to construct virus deletion mutants that can be engineered to obtain attenuated ASFV strains suitable for vaccine approaches. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：168 / 179
页数：12
相关论文
共 50 条
  • [31] Estimation of the transmission dynamics of African swine fever virus within a swine house
    Nielsen, J. P.
    Larsen, T. S.
    Halasa, T.
    Christiansen, L. E.
    EPIDEMIOLOGY AND INFECTION, 2017, 145 (13) : 2787 - 2796
  • [32] Clinical Indicators of Moribundity in Swine Experimentally Inoculated with African Swine Fever Virus
    Hershey, Benjamin J.
    Hagart, Jenna L.
    Havas, Karyn A.
    JOURNAL OF THE AMERICAN ASSOCIATION FOR LABORATORY ANIMAL SCIENCE, 2021, 60 (01): : 96 - 102
  • [33] Characterization of the interaction of African swine fever virus with monocytes and derived macrophage subsets
    Franzoni, Giulia
    Graham, Simon P.
    Dei Giudici, Silvia
    Bonelli, Piero
    Pilo, Giovannantonio
    Anfossi, Antonio G.
    Pittau, Marco
    Nicolussi, Paola S.
    Laddomada, Alberto
    Oggiano, Annalisa
    VETERINARY MICROBIOLOGY, 2017, 198 : 88 - 98
  • [34] Cell Lines for the Development of African Swine Fever Virus Vaccine Candidates: An Update
    Meloni, Dionigia
    Franzoni, Giulia
    Oggiano, Annalisa
    VACCINES, 2022, 10 (05)
  • [35] A sensitive luciferase reporter assay for the detection of infectious African swine fever virus
    Mehinagic, Kemal
    Liniger, Matthias
    Samoilenko, Maksym
    Soltermann, Nick
    Gerber, Markus
    Ruggli, Nicolas
    JOURNAL OF VIROLOGICAL METHODS, 2024, 323
  • [36] African swine fever
    Dixon, L. K.
    Sun, H.
    Roberts, H.
    ANTIVIRAL RESEARCH, 2019, 165 : 34 - 41
  • [37] Multifaceted Immune Responses to African Swine Fever Virus: Implications for Vaccine Development
    Wang, Tao
    Sun, Yuan
    Huang, Shujian
    Qiu, Hua-Ji
    VETERINARY MICROBIOLOGY, 2020, 249
  • [38] Host Innate and Adaptive Immunity Against African Swine Fever Virus Infection
    Zhang, Tianqi
    Lu, Zixun
    Liu, Jia
    Tao, Yang
    Si, Youhui
    Ye, Jing
    Cao, Shengbo
    Zhu, Bibo
    VACCINES, 2024, 12 (11)
  • [39] Bead-Based Multiplex Assay for the Simultaneous Detection of Antibodies to African Swine Fever Virus and Classical Swine Fever Virus
    Aira, Cristina
    Ruiz, Tamara
    Dixon, Linda
    Blome, Sandra
    Rueda, Paloma
    Sastre, Patricia
    FRONTIERS IN VETERINARY SCIENCE, 2019, 6
  • [40] Screening and identification of the dominant antigens of the African swine fever virus
    Xu, Zhaoyang
    Hu, Yifan
    Li, Junbo
    Wang, Ancheng
    Meng, Xin
    Chen, Lingchao
    Wei, Jianchao
    Tong, Wu
    Kong, Ning
    Yu, Lingxue
    Yu, Hai
    Shan, Tongling
    Tong, Guangzhi
    Wang, Guihua
    Zheng, Hao
    FRONTIERS IN VETERINARY SCIENCE, 2023, 10
12345