Integrating conventional microscopy and molecular analysis to analyse the abundance and distribution of four Calanus congeners in the North Atlantic

被引:47
作者
Lindeque, PK
Hay, SJ
Heath, MR
Ingvarsdottir, A
Rasmussen, J
Smerdon, GR
Waniek, JJ
机构
[1] Plymouth Marine Lab, Plymouth PL1 3DH, Devon, England
[2] FRS Marine Lab, Aberdeen AB11 9DB, Scotland
[3] Univ Aberdeen, Dept Zool, Aberdeen AB9 2TN, Scotland
[4] Inst Ostseeforsch, D-18119 Rostock, Germany
基金
英国自然环境研究理事会;
关键词
D O I
10.1093/plankt/fbi115
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
Analysis of the demographic structure of Calanus species in the North Atlantic presents particular difficulties due to the overlapping spatial distributions of four main congeneric species (Calanus finmarchicus, Calanus helgolandicus, Calanus glacialis and Calanus hyperboreus). These species have similar morphologies, making microscopic discrimination only possible between some of the species at late copepodite or adult stages. However, molecular techniques now offer the possibility of screening significant numbers of specimens and unambiguously identifying them to species, regardless of developmental stage. Unfortunately, the processing rate of specimens by molecular methods is still too low to offer a realistic alternative to microscopy for analysis of samples from large field surveys. Here, we outline and test an approach involving the use of molecular methodology in conjunction with conventional microscopy to assess the species assignment of developmental stage abundances of Calanus congeners. Our study has highlighted many important methodological issues. First, it cannot be assumed that the species composition is homogeneous across the development stages; applying proportional species composition of adults to morphologically undistinguishable earlier development stages can result in error. The second important conclusion is that prosome length may be a highly unreliable discriminator of C. finmarchicus and C. glacialis.
引用
收藏
页码:221 / 238
页数:18
相关论文
共 35 条
[11]   TAXONOMIC STATUS OF CALANUS-FINMARCHICUS AND C GLACIALIS (COPEPODA), WITH SPECIAL REFERENCE TO ADULT MALES [J].
FROST, BW .
JOURNAL OF THE FISHERIES RESEARCH BOARD OF CANADA, 1971, 28 (01) :23-&
[12]   Pelagic cod and haddock juveniles on the Faroe plateau: distribution, diets and feeding habitats, 1994-1996 [J].
Gaard, E ;
Reinert, J .
SARSIA, 2002, 87 (03) :193-206
[13]   The food of Norwegian spring-spawning herring in the western Norwegian Sea in relation to the annual cycle of zooplankton [J].
Gislason, A ;
Astthorsson, AS .
SARSIA, 2002, 87 (03) :236-247
[14]   The response of Calanus finmarchicus populations to climate variability in the Northwest Atlantic:: basin-scale forcing associated with the North Atlantic Oscillation [J].
Greene, CH ;
Pershing, AJ .
ICES JOURNAL OF MARINE SCIENCE, 2000, 57 (06) :1536-1544
[15]   PATTERNS OF VARIATION IN THE PROSOME LENGTH OF OVERWINTERING STAGE-V COPEPODITES OF CALANUS-FINMARCHICUS IN THE FIRTH OF CLYDE [J].
GRIGG, H ;
BARDWELL, SJ ;
TYZACK, S .
JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM, 1981, 61 (04) :885-899
[16]   COMPARATIVE OBSERVATIONS ON THE BIOMETRY AND DEVELOPMENT OF CALANUS-FINMARCHICUS AND CALANUS-HELGOLANDICUS IN COPEPODITE STAGE-V, WITH COMMENTS ON OTHER CALANIDAE [J].
GRIGG, H ;
BARDWELL, SJ ;
HOLMES, LJ .
MARINE BIOLOGY, 1987, 96 (02) :253-262
[17]   Distribution of Calanus spp. on and around the Nova Scotia Shelf in April:: evidence for an offshore source of Calanus finmarchicus to the central and western regions [J].
Head, EJH ;
Harris, LR ;
Petrie, B .
CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES, 1999, 56 (12) :2463-2476
[18]   Comparative ecology of over-wintering Calanus finmarchicus in the northern North Atlantic, and implications for life-cycle patterns [J].
Heath, MR ;
Boyle, PR ;
Gislason, A ;
Gurney, WSC ;
Hay, SJ ;
Head, EJH ;
Holmes, S ;
Ingvarsdóttir, A ;
Jónasdóttir, SH ;
Lindeque, P ;
Pollard, RT ;
Rasmussen, J ;
Richards, K ;
Richardson, K ;
Smerdon, G ;
Speirs, D .
ICES JOURNAL OF MARINE SCIENCE, 2004, 61 (04) :698-708
[19]   Comparative analysis of Calanus finmarchicus demography at locations around the Northeast Atlantic [J].
Heath, MR ;
Astthorsson, OS ;
Dunn, J ;
Ellertsen, B ;
Gaard, E ;
Gislason, A ;
Gurney, WSC ;
Hind, AT ;
Irigoien, X ;
Melle, W ;
Niehoff, B ;
Olsen, K ;
Skreslet, S ;
Tande, KS .
ICES JOURNAL OF MARINE SCIENCE, 2000, 57 (06) :1562-1580
[20]  
HIRCHE HJ, 1991, POLAR BIOL, V11, P351