Label-free single-cell protein quantification using a drop-based mix-and-read system

被引：23

作者：

Abbaspourrad, Alireza ^{[1
]}

Zhang, Huidan ^{[1
,2
,3
]}

Tao, Ye ^{[1
,4
]}

Cui, Naiwen ^{[1
]}

Asahara, Haruichi ^{[6
]}

Zhou, Ying ^{[6
]}

Yue, Dongxian ^{[6
]}

Koehler, Stephan A. ^{[1
,5
]}

Ung, Lloyd W. ^{[1
]}

Heyman, John ^{[1
]}

Ren, Yukun ^{[4
]}

Ziblat, Roy ^{[1
]}

Chong, Shaorong ^{[6
]}

Weitz, David A. ^{[1
,5
]}

机构：

[1] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[2] China Med Univ, Minist Publ Hlth, Key Lab Cell Biol, Dept Cell Biol, Shenyang 110001, Peoples R China

[3] China Med Univ, Key Lab Med Cell Biol, Minist Educ, Shenyang 110001, Peoples R China

[4] Harbin Inst Technol, Sch Mech Engn, Harbin 150001, Peoples R China

[5] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

[6] New England Biolabs Inc, Ipswich, MA 01938 USA

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

美国国家科学基金会; 中国国家自然科学基金;

关键词：

IMMUNE; TECHNOLOGIES; DISPLAY; BINDING;

D O I：

10.1038/srep12756

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Quantitative protein analysis of single cells is rarely achieved due to technical difficulties of detecting minute amounts of proteins present in one cell. We develop a mix-and-read assay for drop-based label-free protein analysis of single cells. This high-throughput method quantifies absolute, rather than relative, amounts of proteins and does not involve antibody labeling or mass spectrometry.

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页数：7

相关论文

共 21 条

[1] In vitro display technologies:: novel developments and applications [J].

Amstutz, P ;

Forrer, P ;

Zahnd, C ;

Plückthun, A .

CURRENT OPINION IN BIOTECHNOLOGY, 2001, 12 (04) :400-405

[2] A deep profiler's guide to cytometry [J].

Bendall, Sean C. ;

Nolan, Garry P. ;

Roederer, Mario ;

Chattopadhyay, Pratip K. .

TRENDS IN IMMUNOLOGY, 2012, 33 (07) :323-332

[3] Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum [J].

Bendall, Sean C. ;

Simonds, Erin F. ;

Qiu, Peng ;

Amir, El-ad D. ;

Krutzik, Peter O. ;

Finck, Rachel ;

Bruggner, Robert V. ;

Melamed, Rachel ;

Trejo, Angelica ;

Ornatsky, Olga I. ;

Balderas, Robert S. ;

Plevritis, Sylvia K. ;

Sachs, Karen ;

Pe'er, Dana ;

Tanner, Scott D. ;

Nolan, Garry P. .

SCIENCE, 2011, 332 (6030) :687-696

[4] Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry [J].

Chattopadhyay, Pratip K. ;

Price, David A. ;

Harper, Theresa F. ;

Betts, Michael R. ;

Yu, Joanne ;

Gostick, Emma ;

Perfetto, Stephen P. ;

Goepfert, Paul ;

Koup, Richard A. ;

De Rosa, Stephen C. ;

Bruchez, Marcel P. ;

Roederer, Mario .

NATURE MEDICINE, 2006, 12 (08) :972-977

[5] Single-cell technologies for monitoring immune systems [J].

Chattopadhyay, Pratip K. ;

Gierahn, Todd M. ;

Roederer, Mario ;

Love, J. Christopher .

NATURE IMMUNOLOGY, 2014, 15 (02) :128-135

[6] Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics [J].

Chokkalingam, Venkatachalam ;

Tel, Jurjen ;

Wimmers, Florian ;

Liu, Xin ;

Semenov, Sergey ;

Thiele, Julian ;

Figdor, Carl G. ;

Huck, Wilhelm T. S. .

LAB ON A CHIP, 2013, 13 (24) :4740-4744

[7]

ELIASSON M, 1988, J BIOL CHEM, V263, P4323

[8] Development of quantitative cell-based enzyme assays in microdroplets [J].

Huebner, Ansgar ;

Olguin, Luis F. ;

Bratton, Daniel ;

Whyte, Graeme ;

Huck, Wilhelm T. S. ;

de Mello, Andrew J. ;

Edel, Joshua B. ;

Abell, Chris ;

Hollfelder, Florian .

ANALYTICAL CHEMISTRY, 2008, 80 (10) :3890-3896

[9]

Hughes AJ, 2014, NAT METHODS, V11, P749, DOI [10.1038/NMETH.2992, 10.1038/nmeth.2992]

[10] Droplet Microfluidics-A Tool for Single-Cell Analysis [J].

Joensson, Haakan N. ;

Svahn, Helene Andersson .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 51 (49) :12176-12192