Label-free single-cell protein quantification using a drop-based mix-and-read system

被引:23
作者
Abbaspourrad, Alireza [1 ]
Zhang, Huidan [1 ,2 ,3 ]
Tao, Ye [1 ,4 ]
Cui, Naiwen [1 ]
Asahara, Haruichi [6 ]
Zhou, Ying [6 ]
Yue, Dongxian [6 ]
Koehler, Stephan A. [1 ,5 ]
Ung, Lloyd W. [1 ]
Heyman, John [1 ]
Ren, Yukun [4 ]
Ziblat, Roy [1 ]
Chong, Shaorong [6 ]
Weitz, David A. [1 ,5 ]
机构
[1] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA
[2] China Med Univ, Minist Publ Hlth, Key Lab Cell Biol, Dept Cell Biol, Shenyang 110001, Peoples R China
[3] China Med Univ, Key Lab Med Cell Biol, Minist Educ, Shenyang 110001, Peoples R China
[4] Harbin Inst Technol, Sch Mech Engn, Harbin 150001, Peoples R China
[5] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA
[6] New England Biolabs Inc, Ipswich, MA 01938 USA
基金
美国国家科学基金会; 中国国家自然科学基金;
关键词
IMMUNE; TECHNOLOGIES; DISPLAY; BINDING;
D O I
10.1038/srep12756
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Quantitative protein analysis of single cells is rarely achieved due to technical difficulties of detecting minute amounts of proteins present in one cell. We develop a mix-and-read assay for drop-based label-free protein analysis of single cells. This high-throughput method quantifies absolute, rather than relative, amounts of proteins and does not involve antibody labeling or mass spectrometry.
引用
收藏
页数:7
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