Eicosapentaenoic acid production from Nannochloropsis oceanica CY2 using deep sea water in outdoor plastic-bag type photobioreactors

被引:28
作者
Chen, Chun-Yen [1 ]
Nagarajan, Dillirani [2 ]
Cheah, Wai Yan [3 ]
机构
[1] Natl Cheng Kung Univ, Univ Ctr Biosci & Biotechnol, Tainan 701, Taiwan
[2] Natl Cheng Kung Univ, Dept Chem Engn, Tainan, Taiwan
[3] Univ Malaya, Fac Sci, Inst Biol Sci, Kuala Lumpur 50603, Malaysia
关键词
Algae; EPA; Deep-sea water; Semi-batch; ANAEROBIC-DIGESTION EFFLUENT; LIPID PRODUCTION; FATTY-ACID; OIL PRODUCTION; ENGINEERING STRATEGIES; OLEAGINOUS MICROALGAE; SP EUSTIGMATOPHYCEAE; BIODIESEL PRODUCTION; PAVLOVA-LUTHERI; CO2; FIXATION;
D O I
10.1016/j.biortech.2017.12.102
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
In this study, Nannochloropsis oceanica CY2 was grown in deep-sea water (DSW)-based medium in 5-L plastic bag-type photobioreactors (PBRs) for the autotrophic production of Eicosapentaenoic acid (EPA, 20:5n-3). EPA production of N. oceanica CY2 was stimulated when it was grown in 100% DSW amended with 1.5 g L-1 NaNO3, achieving a EPA content of 3.1% and a biomass concentration of 3.3 g L-1. An outdoor-simulated microalgae cultivation system was also conducted to validate the feasibility of outdoor cultivation of the CY2 strain in plastic bag-type PBRs. Using an inoculum size of 0.6 g/L, the biomass concentration in the PBR culture was 3.5 g L-1, while the EPA content and productivity reached a maximal level of 4.12% and 7.49 mg L-1 d(-1), respectively. When the PBRs were operated on semi-batch mode, the EPA productivity could further increase to 9.9 mg L-1 d(-1) with a stable EPA content of 4.1%.
引用
收藏
页码:1 / 7
页数:7
相关论文
共 43 条
[1]   Allelopathy as a potential strategy to improve microalgae cultivation [J].
Bacellar Mendes, Leonardo Brantes ;
Vermelho, Alane Beatriz .
BIOTECHNOLOGY FOR BIOFUELS, 2013, 6
[2]   Effects of salinity on growth and lipid accumulation of biofuel microalga Nannochloropsis salina and invading organisms [J].
Bartley, Meridith L. ;
Boeing, Wiebke J. ;
Corcoran, Alina A. ;
Holguin, F. Omar ;
Schaub, Tanner .
BIOMASS & BIOENERGY, 2013, 54 :83-88
[3]   LIPID AND BIOMASS PRODUCTION BY THE HALOTOLERANT MICROALGA NANNOCHLOROPSIS-SALINA [J].
BOUSSIBA, S ;
VONSHAK, A ;
COHEN, Z ;
AVISSAR, Y ;
RICHMOND, A .
BIOMASS, 1987, 12 (01) :37-47
[4]   Comparison of Synechocystis sp PCC6803 and Nannochloropsis salina for lipid production using artificial seawater and nutrients from anaerobic digestion effluent [J].
Cai, Ting ;
Ge, Xumeng ;
Park, Stephen Y. ;
Li, Yebo .
BIORESOURCE TECHNOLOGY, 2013, 144 :255-260
[5]   Cultivation of Nannochloropsis sauna using anaerobic digestion effluent as a nutrient source for biofuel production [J].
Cai, Ting ;
Park, Stephen Y. ;
Racharaks, Ratanachat ;
Li, Yebo .
APPLIED ENERGY, 2013, 108 :486-492
[6]   A low-cost culture medium for the production of Nannochloropsis gaditana biomass optimized for aquaculture [J].
Camacho-Rodriguez, J. ;
Ceron-Garcia, M. C. ;
Gonzalez-Lopez, C. V. ;
Fernandez-Sevilla, J. M. ;
Contreras-Gomez, A. ;
Molina-Grima, E. .
BIORESOURCE TECHNOLOGY, 2013, 144 :57-66
[7]   Metabolic relationships between macro- and micronutrients, and the eicosapentaenoic acid and docosahexaenoic acid contents of Pavlova lutheri [J].
Carvalho, AP ;
Pontes, I ;
Gaspar, H ;
Malcata, FX .
ENZYME AND MICROBIAL TECHNOLOGY, 2006, 38 (3-4) :358-366
[8]   A techno-economic analysis of industrial production of marine microalgae as a source of EPA and DHA-rich raw material for aquafeed: Research challenges and possibilities [J].
Chauton, Matilde S. ;
Reitan, Kjell Inge ;
Norsker, Niels Henrik ;
Tveteras, Ragnar ;
Kleivdal, Hans T. .
AQUACULTURE, 2015, 436 :95-103
[9]   Biosequestration of atmospheric CO2 and flue gas-containing CO2 by microalgae [J].
Cheah, Wai Yan ;
Show, Pau Loke ;
Chang, Jo-Shu ;
Ling, Tau Chuan ;
Juan, Joon Ching .
BIORESOURCE TECHNOLOGY, 2015, 184 :190-201
[10]   Enhancing the production of eicosapentaenoic acid (EPA) from Nannochloropsis oceanica CY2 using innovative photobioreactors with optimal light source arrangements [J].
Chen, Chun-Yen ;
Chen, Yu-Chun ;
Huang, Hsiao-Chen ;
Ho, Shih-Hsin ;
Chang, Jo-Shu .
BIORESOURCE TECHNOLOGY, 2015, 191 :407-413