Gonadotropins regulate ovarian germ cell mitosis/meiosis decision in the embryonic chicken

被引：24
作者：
He, Bin
Mi, Yuling
Zhang, Caiqiao
机构：
[1] Zhejiang Univ, Coll Anim Sci, Minist Educ, Key Lab Mol Anim Nutr, Hangzhou 310058, Zhejiang, Peoples R China
[2] Zhejiang Univ, Coll Anim Sci, Dept Vet Med, Hangzhou 310058, Zhejiang, Peoples R China
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2013年 / 370卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
Gonadotropins; Germ cell; Meiotic initiation; Mitosis; MiR181a*; Chicken; FOLLICLE-STIMULATING-HORMONE; NEWLY-HATCHED CHICKS; RETINOIC ACID; MEIOTIC INITIATION; SUPPRESSES MEIOSIS; PROLIFERATION; MICE; RECEPTOR; FSH; SUBPOPULATIONS;
D O I：
10.1016/j.mce.2013.02.010
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Gonadotropins are required for gametogenesis but in embryonic gonads this mechanism is not well understood. Here we use chicken embryos to investigate the mechanism that gonadotropins regulate the ovarian germ cell mitosis/meiosis decision. Treatment with follicle-stimulating hormone (FSH) delayed germ cell meiosis entry and promoted their proliferation. This action was blocked by an aromatase inhibitor. Treatment with luteinizing hormone (LH) accelerated germ cell meiosis entry and promoted transcription of 3 beta HSDII to increase progesterone (P-4) production. In the cultured ovaries, P-4 triggered meiotic initiation in germ cells. MiR181a*, which acts to downregulate the NR6A1 transcript to inhibit the meiotic initiation, was upregulated by FSH and downregulated by LH. Collectively, gonadotropins regulate germ cells mitosis and meiotic initiation through steroid hormones and a miR181a*-mediated pathway. In particularly, FSH delays germ cell meiosis entry and promotes cell proliferation via estrogen while LH accelerates the meiotic initiation via elevated P4 production. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：32 / 41
页数：10
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