Sendai virus defective-interfering genomes and the activation of interferon-beta

被引:171
作者
Strahle, Laura [1 ]
Garcin, Dorninique [1 ]
Kolakofsky, Daniel [1 ]
机构
[1] Univ Geneva, Sch Med, Dept Microbiol & Mol Med, CH-1211 Geneva, Switzerland
关键词
Sendai virus; defective-interfering genome; interferon beta; innate immunity;
D O I
10.1016/j.virol.2006.03.022
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The ability of some Sendai virus stocks to strongly activate IFN beta has long been known to be associated with defective-interfering (DI) genomes. We have compared SeV stocks containing various copyback and internal deletion DI genomes (and those containing only nondefective (ND) genomes) for their ability to activate reporter genes driven by the IFN beta promoter. We found that this property was primarily due to the presence of copyback DI genomes and correlated with their ability to self-anneal and form dsRNA. The level of IFNf activation was found to be proportional to that of DI genome replication and to the ratio of DI to ND genomes during infection. Over-expression of the viral V and C proteins was as effective in blocking the copyback DI-induced activation of the IFN beta promoter as it was in reducing poly-I/C-induced activation, providing evidence that these DI infections activate IFN beta via dsRNA. Infection with an SeV stock that is highly contaminated with copyback DI genomes is thus a very particular way of potently activating IFN beta, presumably by providing plentiful dsRNA under conditions of reduced expression of viral products which block the host antiviral response. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:101 / 111
页数:11
相关论文
共 49 条
[1]   The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-β promoter [J].
Andrejeva, J ;
Childs, KS ;
Young, DF ;
Carlos, TS ;
Stock, N ;
Goodbourn, S ;
Randall, RE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (49) :17264-17269
[2]   MOLECULAR-CLONING OF NATURAL PARAMYXOVIRUS COPY-BACK DEFECTIVE INTERFERING RNAS AND THEIR EXPRESSION FROM DNA [J].
CALAIN, P ;
CURRAN, J ;
KOLAKOFSKY, D ;
ROUX, L .
VIROLOGY, 1992, 191 (01) :62-71
[3]   SYNTHESIS INVITRO OF THE FULL-LENGTH COMPLEMENT OF DEFECTIVE-INTERFERING PARTICLE RNA OF VESICULAR STOMATITIS-VIRUS [J].
CHANDA, PK ;
KANG, CY ;
BANERJEE, AK .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (07) :3927-3931
[4]   THE SENDAI VIRUS P-GENE EXPRESSES BOTH AN ESSENTIAL PROTEIN AND AN INHIBITOR OF RNA-SYNTHESIS BY SHUFFLING MODULES VIA MESSENGER-RNA EDITING [J].
CURRAN, J ;
BOECK, R ;
KOLAKOFSKY, D .
EMBO JOURNAL, 1991, 10 (10) :3079-3085
[5]   Sendai viruses with altered P, V, and W protein expression [J].
Delenda, C ;
Taylor, G ;
Hausmann, S ;
Garcin, D ;
Kolakofsky, D .
VIROLOGY, 1998, 242 (02) :327-337
[6]   Sendai virus and simian virus 5 block activation of interferon-responsive genes: Importance for virus pathogenesis [J].
Didcock, L ;
Young, DF ;
Goodbourn, S ;
Randall, RE .
JOURNAL OF VIROLOGY, 1999, 73 (04) :3125-3133
[7]   MOLECULAR-CLONING AND CHARACTERIZATION OF A SENDAI VIRUS INTERNAL DELETION DEFECTIVE-RNA [J].
ENGELHORN, M ;
STRICKER, R ;
ROUX, L .
JOURNAL OF GENERAL VIROLOGY, 1993, 74 :137-141
[8]   IKKε and TBK1 are essential components of the IRF3 signaling pathway [J].
Fitzgerald, KA ;
McWhirter, SM ;
Faia, KL ;
Rowe, DC ;
Latz, E ;
Golenbock, DT ;
Coyle, AJ ;
Liao, SM ;
Maniatis, T .
NATURE IMMUNOLOGY, 2003, 4 (05) :491-496
[9]   INDUCTION OF INTERFERON IN L-CELLS BY DEFECTIVE-INTERFERING (DI) PARTICLES OF VESICULAR STOMATITIS-VIRUS - LACK OF CORRELATION WITH CONTENT OF [+/-] SNAPBACK RNA [J].
FREY, TK ;
JONES, EV ;
CARDAMONE, JJ ;
YOUNGNER, JS .
VIROLOGY, 1979, 99 (01) :95-102
[10]   Involvement of the leader sequence in Sendai virus pathogenesis revealed by recovery of a pathogenic field isolate from cDNA [J].
Fujii, Y ;
Sakaguchi, T ;
Kiyotani, K ;
Huang, C ;
Fukuhara, N ;
Egi, Y ;
Yoshida, T .
JOURNAL OF VIROLOGY, 2002, 76 (17) :8540-8547