Capsaicin Modulates Proliferation, Migration, and Activation of Hepatic Stellate Cells

被引：19

作者：

Bitencourt, Shanna ^{[1
,2
]}

Mesquita, Fernanda ^{[1
]}

Basso, Bruno ^{[1
]}

Schmid, Julia ^{[1
]}

Ferreira, Gabriela ^{[1
]}

Rizzo, Lucas ^{[3
]}

Bauer, Moises ^{[3
]}

Bartrons, Ramon ^{[4
]}

Ventura, Francesc ^{[4
]}

Luis Rosa, Jose ^{[4
]}

Mannaerts, Inge ^{[2
]}

van Grunsven, Leo Adrianus ^{[2
]}

Oliveira, Jarbas ^{[1
]}

机构：

[1] Pontificia Univ Catolica Rio Grande Sul PUCRS, Lab Pesquisa Biofis Celular & Inflamacao, BR-90619900 Porto Alegre, RS, Brazil

[2] Vrije Univ Brussel, Dept Cell Biol, Liver Cell Biol Lab, Brussels, Belgium

[3] Pontificia Univ Catolica Rio Grande do Sul, Inst Pesquisas Biomed, Lab Imunol Envelhecimento, Porto Alegre, RS, Brazil

[4] Univ Barcelona, Dept Ciencies Fisiol 2, Barcelona, Spain

来源：

CELL BIOCHEMISTRY AND BIOPHYSICS | 2014年 / 68卷 / 02期

关键词：

Hepatic stellate cell; Capsaicin; Proliferation; Migration; Activation; CYCLE ARREST; IN-VITRO; LIVER; APOPTOSIS; GROWTH; INHIBITION; INDUCTION; MATRIX; GAMMA;

D O I：

10.1007/s12013-013-9719-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Capsaicin, the active component of chili pepper, has been reported to have antiproliferative and anti-inflammatory effects on a variety of cell lines. In the current study, we aimed to investigate the effects of capsaicin during HSC activation and maintenance. Activated and freshly isolated HSCs were treated with capsaicin. Proliferation was measured by incorporation of EdU. Cell cycle arrest and apoptosis were investigated using flow cytometry. The migratory response to chemotactic stimuli was evaluated by a modified Boyden chamber assay. Activation markers and inflammatory cytokines were determined by qPCR, immunocytochemistry, and flow cytometry. Our results show that capsaicin reduces HSC proliferation, migration, and expression of profibrogenic markers of activated and primary mouse HSCs. In conclusion, the present study shows that capsaicin modulates proliferation, migration, and activation of HSC in vitro.

引用

页码：387 / 396

页数：10

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