Covalent attachment of enzymes and other proteins to the smart polymer, poly(N-isopropylacrylamide) [poly (NIPAAm)], has been widely used as a method for the preparation of thermosensitive protein conjugates. In the present study, reversible soluble-insoluble polymer-enzyme conjugates were prepared by conjugating a copolymer of NIPAAm with 5-mol % of 6-acrylaminohexanoic acid to trypsin by the carbodiimide-NHS (N-hydroxysuccinimide) coupling method. Four bioconjugates with different units of enzyme coupled to the matrix were prepared. Increased enzymatic activity in terms of high effectiveness factor (in the range of 3-5) was found in the conjugates. Kinetic parameters for the immobilized and free enzyme were determined. The V-max/K-m value of the enzyme significantly increased on immobilization by the factors in the range of 12-28. The immobilized enzyme also showed stability to autolysis at 50 degrees C.
机构:
Ocean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R ChinaOcean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China
Wu, Zhiqiang
Jiang, Guoliang
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Ocean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R ChinaOcean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China
Jiang, Guoliang
Wang, Ning
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Ocean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R ChinaOcean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China
Wang, Ning
Wang, Jun
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Ocean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R ChinaOcean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China
Wang, Jun
Chen, Shuang
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Ocean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R ChinaOcean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China
Chen, Shuang
Xu, Zinan
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Ocean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R ChinaOcean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China
机构:
Ocean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R ChinaOcean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China
Wu, Zhiqiang
Jiang, Guoliang
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Ocean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R ChinaOcean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China
Jiang, Guoliang
Wang, Ning
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Ocean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R ChinaOcean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China
Wang, Ning
Wang, Jun
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Ocean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R ChinaOcean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China
Wang, Jun
Chen, Shuang
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Ocean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R ChinaOcean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China
Chen, Shuang
Xu, Zinan
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h-index: 0
机构:
Ocean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R ChinaOcean Univ China, Marine Biol Mat Lab, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R China