Temperature effect on nervous necrosis virus infection in grouper cell line and in grouper larvae

被引:54
作者
Chi, SC [1 ]
Lin, SC
Su, HM
Hu, WW
机构
[1] Natl Taiwan Univ, Dept Zool, Taipei 10764, Taiwan
[2] Taiwan Fisheries Res Inst, Tungkang Marine Lab, Pingtung, Taiwan
关键词
fish nodavirus; grouper; nervous necrosis virus; temperature effect;
D O I
10.1016/S0168-1702(99)00063-5
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This preliminary study elucidates the in vitro and in vivo effects of temperature on grouper nervous necrosis virus (GNNV) infection. A novel continuous cell line derived from the fin tissue of a grouper (Epinephelus coioides, Hamilton), named as GF-1 cell line, was used. Cytopathic effect was observed in GNNV-infected GF-1 cells incubated at 24-32 degrees C after viral adsorption, but not at 20 degrees C or 37 degrees C even though the viral adsorption temperature was 28 degrees C. Viral protein could be detected in the pellets of GNNV-infected GF-1 cells cultured at 20-32 degrees C, but not at 37 degrees C. In a challenge test, GNNV-challenged larvae which were maintained at a constant 28 degrees C began to die 1 day post challenge (p.c.) with a death rate of 80%. Mortality reached 100% by 50 h p.c., while the mortality of negative control fish was only 5%. The cumulative mortality of GNNV-challenged larvae at ambient temperature, i.e. 28 degrees C at noon and 24 degrees C at midnight, was 10% 1 day p.c., and increased to 100% by 80 h p.c. Based on the results, we concluded that temperature plays an important role in GNNV infection and pathogenicity. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:107 / 114
页数:8
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